Molecular characterization of metallothionein from white shrimp, Litopenaeus vannamei and its expression response to salinity stress
文献类型：期刊论文
| 作者 | Wang, YH ; Zhao, Z ; Zhang, LP ; Hu, CQ ; Ren, CH ; Yuan, LH |
| 刊名 | MARINE BIOLOGY RESEARCH
 |
| 出版日期 | 2014 |
| 卷号 | 10期号:7页码:731-737 |
| ISSN号 | 1745-1000 |
| 关键词 | Gene expression Litopenaeus vannamei metallothionein salinity stress subcellular localization |
| 通讯作者 | cqhu@scsio.ac.cn |
| 中文摘要 | The metallothioneins (MTs) are conserved and multi-functional proteins which play crucial roles in the transportation of essential trace elements and the detoxification of harmful metallic elements. A novel MT homologue (accession no. JN707684) was isolated from the Litopenaeus vannamei hypersalinity subtractive library and used to carry out tissue-specific expression under varying salinity stress. The complete cDNA sequence of LvMT contains a 177 bp open reading frame encoding 58 amino acids, a 66 bp 5-UTR, and a 117 bp 3-UTR. The LvMT was expressed in the highest peak in the hepatopancreas under hyposalinity (3 g l(-1)) (19.43-fold, p < 0.01) and hypersalinity (40 g l(-1)) (22.3-fold, p < 0.01) stress for 6 h. A phylogenetic analysis revealed that LvMT is similar to other crustacean MT proteins. The LvMT was distributed in both the cytoplasm and the nucleus of HeLa and EPC cells. |
| 学科主题 | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| 资助信息 | National High Technology Research and Development Program of China (863 Program) [2012AA10A404-4]; Science & Technology Promoting Project from the Oceanic & Fishery Bureau of Guangdong Province [A201001A02, A201101B01, A201201B02]; Science and Technology Program of Guangdong Province [2012B091100270] |
| 收录类别 | SCI |
| 原文出处 | TAYLOR & FRANCIS AS |
| WOS记录号 | WOS:000333879100007 |
| 公开日期 | 2014-12-11 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/10403]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, YH,Zhao, Z,Zhang, LP,et al. Molecular characterization of metallothionein from white shrimp, Litopenaeus vannamei and its expression response to salinity stress[J]. MARINE BIOLOGY RESEARCH,2014,10(7):731-737. |
| APA | Wang, YH,Zhao, Z,Zhang, LP,Hu, CQ,Ren, CH,&Yuan, LH.(2014).Molecular characterization of metallothionein from white shrimp, Litopenaeus vannamei and its expression response to salinity stress.MARINE BIOLOGY RESEARCH,10(7),731-737. |
| MLA | Wang, YH,et al."Molecular characterization of metallothionein from white shrimp, Litopenaeus vannamei and its expression response to salinity stress".MARINE BIOLOGY RESEARCH 10.7(2014):731-737. |
入库方式： OAI收割
来源：南海海洋研究所
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