Molecular characterization and analysis of a putative 5-HT receptor involved in reproduction process of the pearl oyster Pinctada fucata
文献类型：期刊论文
| 作者 | Wang, Q ; He, MX |
| 刊名 | GENERAL AND COMPARATIVE ENDOCRINOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 204页码:71-79 |
| 关键词 | 5-HT receptor Oocyte maturation Spawning Pinctada fucata |
| ISSN号 | 0016-6480 |
| 通讯作者 | hmx@scsio.ac.cn |
| 中文摘要 | 5-HT (5-hydroxytryptamine; serotonin) has been linked to a variety of biological roles including gonad maturation and sequential spawning in bivalve molluscs. To gain a better understanding of the effects of 5-HT on developmental regulation in the pearl oyster Pinctada fucata, the isolation, cloning, and expression of the 5-HT receptor was investigated in this study. A full-length cDNA (2541 bp) encoding a putative 5-HT receptor (5-HTpf) of 471 amino acids was isolated from the ovary of the pearl oyster. It shared 71% and 51% homology, respectively, with the Crassostrea gigas 5-HT receptor and the Aplysia californica 5-HT1ap. The 5-HTpf sequence possessed the typical characteristics of seven transmembrane domains and a long third inner loop. Phylogenetic analysis also indicated that 5-HTpf was classified into the 5-HT1 subtype together with other invertebrate 5-HT1 receptors. Quantitative RT-PCR showed that 5-HTpf is widely expressed in all tissues tested, is involved in the gametogenesis cycle, embryonic and larval development stages, and expression is induced by E-2 in ovarian tissues. These results suggest that 5-HTpf is involved in the reproductive process, specifically in the induction of oocyte maturation and spawning of P. fucata. (C) 2014 Elsevier Inc. All rights reserved. |
| 学科主题 | Endocrinology & Metabolism |
| 收录类别 | SCI |
| 资助信息 | Marine Fishery Science and Technology Promotion Program of Guangdong Province, China [A201201A05, A201301A03]; National Science and Technology Program of China [2012AA10A410] |
| 原文出处 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| WOS记录号 | WOS:000341616200009 |
| 公开日期 | 2014-12-11 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/10409]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, Q,He, MX. Molecular characterization and analysis of a putative 5-HT receptor involved in reproduction process of the pearl oyster Pinctada fucata[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY,2014,204:71-79. |
| APA | Wang, Q,&He, MX.(2014).Molecular characterization and analysis of a putative 5-HT receptor involved in reproduction process of the pearl oyster Pinctada fucata.GENERAL AND COMPARATIVE ENDOCRINOLOGY,204,71-79. |
| MLA | Wang, Q,et al."Molecular characterization and analysis of a putative 5-HT receptor involved in reproduction process of the pearl oyster Pinctada fucata".GENERAL AND COMPARATIVE ENDOCRINOLOGY 204(2014):71-79. |
入库方式： OAI收割
来源：南海海洋研究所
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