Cloning and characterization of a novel GH44 family endoglucanase from mangrove soil metagenomic library
文献类型：期刊论文
| 作者 | Mai, ZM ; Su, HF ; Yang, J ; Huang, SJ ; Zhang, S |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2014 |
| 卷号 | 36期号:8页码:1701-1709 |
| 关键词 | Cellulase Mangrove Metagenomic library Organic solvent-resistant Salt-tolerant |
| ISSN号 | 0141-5492 |
| 通讯作者 | maizhimao@163.com ; suhongfei17@126.com ; yangjian@scsio.ac.cn ; huangsijun@scsio.ac.cn ; zhsimd@scsio.ac.cn |
| 中文摘要 | A novel endoglucanase gene, mgcel44, was isolated from a mangrove soil metagenomic library by functional-based screening. It encodes a 648-aa peptide with a catalytic domain of glycosyl hydrolase family 44. The deduced amino acid sequence of mgcel44 shares less than 50 % identity with endoglucanases in GenBank database. mgcel44 was cloned and overexpressed in Escherichia coli. The recombinant enzyme, MgCel44, has a molecular mass of 70.8 kDa as determined by SDS-PAGE. Its optimal pH and temperature for activity were 6 and 45 A degrees C, respectively. It was highly active at 25-45 A degrees C and pH 5-8. Its activity was enhanced in 0.5 M NaCl by > 1.6-fold and stable up to 1.5 M NaCl. MgCel44 was resistant to several organic solvents and had high activity at 15 % (v/v) solvent after incubating for 24 h at 25 A degrees C. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China (973 Program) [2010CB 833801]; National Natural Science Foundation of China [41230962] |
| 原文出处 | SPRINGER |
| WOS记录号 | WOS:000339004900018 |
| 公开日期 | 2014-12-11 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/10417]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Mai, ZM,Su, HF,Yang, J,et al. Cloning and characterization of a novel GH44 family endoglucanase from mangrove soil metagenomic library[J]. BIOTECHNOLOGY LETTERS,2014,36(8):1701-1709. |
| APA | Mai, ZM,Su, HF,Yang, J,Huang, SJ,&Zhang, S.(2014).Cloning and characterization of a novel GH44 family endoglucanase from mangrove soil metagenomic library.BIOTECHNOLOGY LETTERS,36(8),1701-1709. |
| MLA | Mai, ZM,et al."Cloning and characterization of a novel GH44 family endoglucanase from mangrove soil metagenomic library".BIOTECHNOLOGY LETTERS 36.8(2014):1701-1709. |
入库方式： OAI收割
来源：南海海洋研究所
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