The asymmetric membrane structure of erythrocytes from Crucian carp studied by atomic force microscopy
文献类型：期刊论文
| 作者 | Tian, YM ; Cai, MJ ; Zhao, WD ; Wang, SW ; Qin, QW ; Wang, HD |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2014 |
| 卷号 | 59期号:21页码:2582-2587 |
| 关键词 | Atomic force microscope Crucian carp Erythrocyte Membrane structure Asymmetry |
| ISSN号 | 1001-6538 |
| 通讯作者 | hdwang@ciac.ac.cn |
| 中文摘要 | Cell membranes play a key role in cellular activities. Fish erythrocytes, prototype of the nucleated erythrocytes of lower vertebrates, are predominantly oval, biconvex discs with elliptical nucleus and much larger in size than human erythrocytes. In attempts to disclose the correlation of membrane structure between fish and human erythrocytes, we used in situ atomic force microscope (AFM) combined with single-molecule force spectroscopy to study the membrane structure of Crucian carp erythrocytes under quasi-native conditions. Our results revealed the asymmetric distribution of proteins in Crucian carp erythrocyte membrane: the outer leaflet of membrane is rather smooth without any proteins, whereas the inner leaflet of membrane is very rough with dense proteins. The asymmetry of fish erythrocyte membrane structure fits well with the semi-mosaic model of human erythrocyte membrane structure. This similarity of membrane structure between human and fish erythrocytes extends the semi-mosaic model of erythrocytes membrane structure to a wider range of species. |
| 学科主题 | Science & Technology - Other Topics |
| 收录类别 | SCI |
| 资助信息 | Ministry of Science and Technology of China [2011CB933600]; National Natural Science Foundation of China [21373200, 31330082, 21203177] |
| 原文出处 | SCIENCE PRESS |
| WOS记录号 | WOS:000338127100012 |
| 公开日期 | 2014-12-11 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/10423]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Tian, YM,Cai, MJ,Zhao, WD,et al. The asymmetric membrane structure of erythrocytes from Crucian carp studied by atomic force microscopy[J]. CHINESE SCIENCE BULLETIN,2014,59(21):2582-2587. |
| APA | Tian, YM,Cai, MJ,Zhao, WD,Wang, SW,Qin, QW,&Wang, HD.(2014).The asymmetric membrane structure of erythrocytes from Crucian carp studied by atomic force microscopy.CHINESE SCIENCE BULLETIN,59(21),2582-2587. |
| MLA | Tian, YM,et al."The asymmetric membrane structure of erythrocytes from Crucian carp studied by atomic force microscopy".CHINESE SCIENCE BULLETIN 59.21(2014):2582-2587. |
入库方式： OAI收割
来源：南海海洋研究所
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