Involvement of SgvP in Carbon-Sulfur Bond Formation during Griseoviridin Biosynthesis
文献类型：期刊论文
| 作者 | Xie, YC ; Li, QL ; Song, YX ; Ma, JY ; Ju, JH |
| 刊名 | CHEMBIOCHEM
 |
| 出版日期 | 2014 |
| 卷号 | 15期号:8页码:1183-1189 |
| 关键词 | antibiotics biosynthesis carbon-sulfur bonds griseoviridin P450 monooxygenases viridogrisein |
| ISSN号 | 1439-4227 |
| 通讯作者 | jju@scsio.ac.cn |
| 中文摘要 | Griseoviridin (GV) is an A-type streptogramin antibiotic displaying antimicrobial activity and acting synergistically with viridogrisein (VG). Bioinformatic analyses reveal SgvP as the sole cytochrome P450 enzyme in the GV/VG gene cluster. To explore the role of SgvP in the GV/VG pathway, we inactivated the sgvP gene. The resulting sgvP mutant generated two new products: GV-1 and GV-2, both lacking the CS bridge. In trans complementation of the sgvP gene into the sgvP mutant strain partially restores GV production. Feeding [1-13C]-labeled cysteine to the wild-type strain led to enrichment of C-7 in the GV scaffold, thus verifying that the CS bond in GV is formed through direct coupling of the free SH group provided by the side chain of cysteine. The above results highlight the significance of SgvP in CS bond formation in griseoviridin biosynthesis. |
| 学科主题 | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy |
| 收录类别 | SCI |
| 资助信息 | NSFC [21172231]; MOST [2010CB833805, 2012AA092104]; Knowledge Innovation Program of CAS [KSCX2-EW-G-12] |
| 原文出处 | WILEY-V C H VERLAG GMBH |
| WOS记录号 | WOS:000337582200017 |
| 公开日期 | 2014-12-11 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/10438]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Xie, YC,Li, QL,Song, YX,et al. Involvement of SgvP in Carbon-Sulfur Bond Formation during Griseoviridin Biosynthesis[J]. CHEMBIOCHEM,2014,15(8):1183-1189. |
| APA | Xie, YC,Li, QL,Song, YX,Ma, JY,&Ju, JH.(2014).Involvement of SgvP in Carbon-Sulfur Bond Formation during Griseoviridin Biosynthesis.CHEMBIOCHEM,15(8),1183-1189. |
| MLA | Xie, YC,et al."Involvement of SgvP in Carbon-Sulfur Bond Formation during Griseoviridin Biosynthesis".CHEMBIOCHEM 15.8(2014):1183-1189. |
入库方式： OAI收割
来源：南海海洋研究所
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