Singapore grouper iridovirus-encoded semaphorin homologue (SGIV-sema) contributes to viral replication, cytoskeleton reorganization and inhibition of cellular immune responses
文献类型：期刊论文
| 作者 | Yan, Y ; Cui, HC ; Guo, CY ; Wei, JG ; Huang, YH ; Li, LL ; Qin, QW |
| 刊名 | JOURNAL OF GENERAL VIROLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 95页码:1144-1155 |
| ISSN号 | 0022-1317 |
| 通讯作者 | qinqw@scsio.ac.cn |
| 中文摘要 | Semaphorins are a large, phylogenetically conserved family of proteins that are involved in a wide range of biological processes including axonal steering, organogenesis, neoplastic transformation, as well as immune responses. In this study, a novel semaphorin homologue gene belonging to the Singapore grouper iridovirus (SGIV), ORF155R (termed SGIV-sema), was cloned and characterized. The coding region of SGIV-sema is 1728 bp in length, encoding a predicted protein with 575 aa. SGIV-sema contains a similar to 370 aa N-terminal Sema domain, a conserved plexin-semaphorin-integrin (PSI) domain, and an immunoglobulin (Ig)-like domain near the C terminus. SGIV-sema is an early gene product during viral infection and predominantly distributed in the cytoplasm with a speckled and clubbed pattern of appearance. Functionally, SGIV-sema could promote viral replication during SGIV infection in vitro, with no effect on the proliferation of host cells. Intriguingly, ectopically expressed SGIV-sema could alter the cytoskeletal structure of fish cells, characterized by a circumferential ring of microtubules near the nucleus and a disrupted microfilament organization. Furthermore, SGIV-sema was able to attenuate the cellular immune response, as demonstrated by decreased expression of inflammation/immune-related genes such as IL-8, IL-15, TNF-alpha and mediator of IRF3 activation (MITA), in SGIV-sema-expressing cells before and after SGIV infection. Ultimately, our study identified a novel, functional SGIV gene that could regulate cytoskeletal structure, immune responses and facilitate viral replication. |
| 学科主题 | Biotechnology & Applied Microbiology ; Virology |
| 资助信息 | National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [41206137, 31330082]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201116, KZCX2-EW-Q213] |
| 收录类别 | SCI |
| 原文出处 | SOC GENERAL MICROBIOLOGY |
| WOS记录号 | WOS:000338177900016 |
| 公开日期 | 2014-12-11 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/10441]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Yan, Y,Cui, HC,Guo, CY,et al. Singapore grouper iridovirus-encoded semaphorin homologue (SGIV-sema) contributes to viral replication, cytoskeleton reorganization and inhibition of cellular immune responses[J]. JOURNAL OF GENERAL VIROLOGY,2014,95:1144-1155. |
| APA | Yan, Y.,Cui, HC.,Guo, CY.,Wei, JG.,Huang, YH.,...&Qin, QW.(2014).Singapore grouper iridovirus-encoded semaphorin homologue (SGIV-sema) contributes to viral replication, cytoskeleton reorganization and inhibition of cellular immune responses.JOURNAL OF GENERAL VIROLOGY,95,1144-1155. |
| MLA | Yan, Y,et al."Singapore grouper iridovirus-encoded semaphorin homologue (SGIV-sema) contributes to viral replication, cytoskeleton reorganization and inhibition of cellular immune responses".JOURNAL OF GENERAL VIROLOGY 95(2014):1144-1155. |
入库方式： OAI收割
来源：南海海洋研究所
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