Molecular cloning and characterization of two types of I kappa B alpha orthologues in orange-spotted grouper, Epinephelus coioides
文献类型：期刊论文
| 作者 | Gao, R ; Huang, YH ; Huang, XH ; Guan, LY ; Wei, SN ; Zhou, YC ; Qin, QW |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 38期号:1页码:101-110 |
| 关键词 | Epinephelus coioides EcI kappa B alpha A EcI kappa B alpha B NF-kappa B SGIV |
| ISSN号 | 1050-4648 |
| 通讯作者 | zychnu@163.com ; qinqw@scsio.ac.cn |
| 中文摘要 | Inhibitors of kappa B (I kappa Bs) are the members of primary regulators of NF-kappa B, which can inhibit NF-kappa B activity by blocking the NF-kappa B in an inactive state in the cytoplasm. In this study, two types of IKB alpha orthologues (EcI kappa B alpha A and EcI kappa B alpha B) from orange-spotted grouper, Epinephelus coioides, were cloned and characterized. EcI kappa B alpha A and EcIKB alpha B encoded putative proteins containing 308 and 318 amino acids, which shared 59% and 53% identity to I kappa B alpha A and I kappa B alpha B of Danio rerio, respectively. Amino acid sequence alignment showed that both EcI kappa B alpha A and EcI kappa B alpha B contained a conserved degradation motif DSGLDS in the N-terminal region and a PEST sequence in the C-terminal region. In addition, EcI kappa B alpha A and EcI kappa B alpha B contained 5 and 6 ankcyrin repeats, respectively. The genomic DNA of EcI kappa B alpha A and EcI kappa B alpha B consisted of 6 exons and 5 introns. Both of their transcripts were widely distributed in different tissues, and the expression levels were different in response to various stimuli, including lipopolysaccharide (LPS), Vibrio alginolyticus and Singapore grouper iridovirus (SGIV). Dual-luciferase reporter assay suggested that both EcI kappa B alpha A and EcI kappa B alpha B were able to inhibit Ecc-Rel and Ecp65 induced NF-kappa B promoter activity in grouper spleen (GS) cells. Subcellular localization analysis showed that EcI kappa B alpha B was present predominantly in the cytoplasm, while EclicB alpha A was distributed throughout both the nucleus and the cytoplasm. Furthermore, overexpression of EcI kappa B alpha A and EcI kappa B alpha B in GS cells inhibited the viral gene transcriptions of MCP, ORF019 and ORF162 of SGIV. Taken together, our findings suggested that both EcI kappa B alpha A and EcI kappa B alpha B were involved in grouper innate immunity against virus. (C) 2014 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China (973) [2012CB114402, 2012CB114404]; Natural Science Foundation of China [31172437, 31372566, 31330082]; Knowledge Innovation Program of the Chinese Academy of Sciences [KZCX2-EW-Q213] |
| 原文出处 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| WOS记录号 | WOS:000336353700014 |
| 公开日期 | 2014-12-11 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/10444]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Gao, R,Huang, YH,Huang, XH,et al. Molecular cloning and characterization of two types of I kappa B alpha orthologues in orange-spotted grouper, Epinephelus coioides[J]. FISH & SHELLFISH IMMUNOLOGY,2014,38(1):101-110. |
| APA | Gao, R.,Huang, YH.,Huang, XH.,Guan, LY.,Wei, SN.,...&Qin, QW.(2014).Molecular cloning and characterization of two types of I kappa B alpha orthologues in orange-spotted grouper, Epinephelus coioides.FISH & SHELLFISH IMMUNOLOGY,38(1),101-110. |
| MLA | Gao, R,et al."Molecular cloning and characterization of two types of I kappa B alpha orthologues in orange-spotted grouper, Epinephelus coioides".FISH & SHELLFISH IMMUNOLOGY 38.1(2014):101-110. |
入库方式： OAI收割
来源：南海海洋研究所
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