A Novel Lipid Extraction Method from Wet Microalga Picochlorum sp at Room Temperature
文献类型：期刊论文
| 作者 | Yang, FF ; Xiang, WZ ; Sun, XM ; Wu, HL ; Li, T ; Long, LJ |
| 刊名 | MARINE DRUGS
 |
| 出版日期 | 2014 |
| 卷号 | 12期号:3页码:1258-1270 |
| 关键词 | lipid extraction wet microalga Picochlorum sp. ethanol biofuel |
| ISSN号 | 1660-3397 |
| 通讯作者 | ycuyang@163.com ; xwz@scsio.ac.cn ; sunxm@scsio.ac.cn ; hlwu@scsio.ac.cn ; taoli@scsio.ac.cn ; longlj@scsio.ac.cn |
| 中文摘要 | A novel method using ethanol was proposed for extracting lipids from wet microalga Picochlorum sp. at room temperature and pressure. In this study, Central Composite design (CCD) was applied to investigate the optimum conditions of lipid extraction. The results revealed that the solvent to biomass ratio had the largest effect on lipid extraction efficiency, followed by extraction time and temperature. A high lipid extraction yield (33.04% of the dry weight) was obtained under the following extraction conditions: 5 mL solvents per gram of wet biomass for 37 min with gentle stirring at room temperature. The extraction yield was comparable to that obtained by the widely used Bligh-Dyer method. Furthermore, no significant differences in the distribution of lipid classes and fatty acid composition were observed according to different extraction methods. In conclusion, these results indicated that the proposed procedure using ethanol could extract lipids from wet biomass efficiently and had giant potential for lipid extraction at large scale. |
| 学科主题 | Pharmacology & Pharmacy |
| 收录类别 | SCI |
| 资助信息 | Ocean Public Welfare Scientific Research Project [201305018-3]; Funds for Marine Renewable Energy [GHME2011SW04]; Chinese Academy of Sciences [KSCX2-YW-R-093] |
| 原文出处 | MDPI AG |
| WOS记录号 | WOS:000335752800006 |
| 公开日期 | 2014-12-11 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/10463]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Yang, FF,Xiang, WZ,Sun, XM,et al. A Novel Lipid Extraction Method from Wet Microalga Picochlorum sp at Room Temperature[J]. MARINE DRUGS,2014,12(3):1258-1270. |
| APA | Yang, FF,Xiang, WZ,Sun, XM,Wu, HL,Li, T,&Long, LJ.(2014).A Novel Lipid Extraction Method from Wet Microalga Picochlorum sp at Room Temperature.MARINE DRUGS,12(3),1258-1270. |
| MLA | Yang, FF,et al."A Novel Lipid Extraction Method from Wet Microalga Picochlorum sp at Room Temperature".MARINE DRUGS 12.3(2014):1258-1270. |
入库方式： OAI收割
来源：南海海洋研究所
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