IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis
文献类型：期刊论文
| 作者 | Fu, JP ; Chen, SN ; Zou, PF ; Huang, B ; Guo, Z ; Zeng, LB ; Qin, QW ; Nie, P |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 43期号:1页码:87-95 |
| 关键词 | IFN-gamma IFN-gamma stimulated gene Iridovirus Antiviral activity Turtle |
| ISSN号 | 0145-305X |
| 通讯作者 | pinnie@ihb.ac.cn |
| 中文摘要 | The IFN-gamma gene was identified in a turtle, the Chinese soft-shelled turtle, Pelodiscus sinensis, with its genome consisting of 4 exons and 3 introns. The deduced amino acid sequence of this gene contains a signal peptide, an IFN-gamma family signature motif (130)IQRKAVNELFPT, an NLS motif (KRKR)-K-155 and three potential N-glycosylation sites. As revealed by real-time quantitative PCR, the gene was constitutively expressed in all tested organs/tissues, with higher level observed in blood, intestine and thymus. An induced expression of IFN-gamma at mRNA level was observed in peripheral blood leucocytes (PBLs) in response to in vitro stimulation of LPS and PolyI:C. The overexpression of IFN-gamma in the Chinese soft-shelled turtle artery (STA) cell line resulted in the increase in the expression of transcriptional regulators, such as IRF1, IRF7 and STAT1, and antiviral genes, such as Mx, PKR, implying possibly the existence of a conserved signalling network and role for IFN-gamma in the turtle. Furthermore, the infection of soft-shelled turtle iridovirus (STIV) in the cell line transfected with IFN-gamma may cause the cell death as demonstrated with the elevated lactate dehydrogenase (LDH) level and cell mortality. However, the mechanism involved in the antiviral activity may require further investigation. (C) 2013 Elsevier Ltd. All rights reserved. |
| 学科主题 | Immunology ; Zoology |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [30230075]; National Basic Research Program (973 Program) of China [2009CB118703] |
| 原文出处 | ELSEVIER SCI LTD |
| WOS记录号 | WOS:000331479800012 |
| 公开日期 | 2014-12-11 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/10468]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Fu, JP,Chen, SN,Zou, PF,et al. IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2014,43(1):87-95. |
| APA | Fu, JP.,Chen, SN.,Zou, PF.,Huang, B.,Guo, Z.,...&Nie, P.(2014).IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,43(1),87-95. |
| MLA | Fu, JP,et al."IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 43.1(2014):87-95. |
入库方式： OAI收割
来源：南海海洋研究所
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