ULTRASONIC-ASSISTED PRODUCTION OF ANTIOXIDATIVE POLYSACCHARIDES FROM Crassostrea hongkongensis
文献类型：期刊论文
| 作者 | Cai, BN ; Pan, JY ; Wan, P ; Chen, DK ; Long, SJ ; Sun, HL |
| 刊名 | PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 44期号:7页码:708-724 |
| 关键词 | antioxidant Crassostrea hongkongensis enzymolysis polysaccharides ultrasonic |
| ISSN号 | 1082-6068 |
| 通讯作者 | jypan@scsio.ac.cn |
| 中文摘要 | The beneficial effects of oyster extract against various disorders and diseases induced by oxidative stress have aroused great interest. In this article, ultrasonic-assisted enzymolysis was employed to produce polysaccharides of Crassostrea hongkongensis (CHP) and their antioxidant activity was investigated. A single-factor experiment and then a four-factor, three-level Box-Behnken design were first used to optimize ultrasonic extraction for polysaccharides. On the basis of ridge analysis, the optimum conditions are obtained as ultrasonic treatment time of 24 min, power of 876 W, temperature of 49 degrees C, and material-solvent ratio of 1:6 (w/v). It is found that ultrasound pretreatment before protease hydrolysis was a great help to improve CHP yield and purity, especially more favorable with flavorzyme, neutrase, alcalase, and pepsin. Furthermore, the polysaccharide fraction, which was obtained by ultrasonic pretreatment and then alcalase hydrolysis at the conditions of 3000 U/g, 55 degrees C, pH 8.0, for 4 hr, exhibited an obvious scavenging effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical (98.48 +/- 0.55% and 99.20 +/- 0.12%, respectively) and a lenoleic acid peroxidation inhibition effect (85.48 +/- 0.65%) at a concentration of 5.0 mg/mL. These results reveal the potential application of CHP in functional food and nutraceuticals. |
| 学科主题 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 收录类别 | SCI |
| 资助信息 | Project of National Key Technology Research and Development Program for the 12th Five-year Plan [2012BAD33B10]; Public Science and Technology Research Funds Projects of Ocean [201305018-2]; Chinese Academy of Sciences [KZCX2-EW-Q214, 2011B090300057]; National Science Foundation for Young Scientists of China [31101271]; Guangdong Province [2011B090300057]; Frontier Science Program for Young Scientists of South China Sea Institute of Oceanology, Chinese Academy of Science [SQ201017] |
| 原文出处 | TAYLOR & FRANCIS INC |
| WOS记录号 | WOS:000338028600006 |
| 公开日期 | 2014-12-11 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/10488]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Cai, BN,Pan, JY,Wan, P,et al. ULTRASONIC-ASSISTED PRODUCTION OF ANTIOXIDATIVE POLYSACCHARIDES FROM Crassostrea hongkongensis[J]. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,2014,44(7):708-724. |
| APA | Cai, BN,Pan, JY,Wan, P,Chen, DK,Long, SJ,&Sun, HL.(2014).ULTRASONIC-ASSISTED PRODUCTION OF ANTIOXIDATIVE POLYSACCHARIDES FROM Crassostrea hongkongensis.PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,44(7),708-724. |
| MLA | Cai, BN,et al."ULTRASONIC-ASSISTED PRODUCTION OF ANTIOXIDATIVE POLYSACCHARIDES FROM Crassostrea hongkongensis".PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY 44.7(2014):708-724. |
入库方式： OAI收割
来源：南海海洋研究所
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