Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100
文献类型：期刊论文
| 作者 | Zhang, Yu1,2 ; Xu, Jing-Liang1; Li, Dong1; Yuan, Zhen-Hong1
|
| 刊名 | biocatalysis and biotransformation
 |
| 出版日期 | 2010-12-01 |
| 卷号 | 28期号:5-6页码:313-319 |
| 关键词 | Immobilized cellulase Eudragit L-100 N-hydroxysuccinimide carbodiimide coupling smart biocatalysis |
| ISSN号 | 1024-2422 |
| 产权排序 | [zhang, yu; xu, jing-liang; li, dong; yuan, zhen-hong] chinese acad sci, guangzhou inst energy & convers, key lab renewable energy & gas hydrate, guangzhou 510640, peoples r china; [zhang, yu] chinese acad sci, grad univ, beijing, peoples r china |
| 通讯作者 | yuanzh@ms.giec.ac.cn |
| 中文摘要 | to prepare a smart biocatalyst, cellulase was immobilized on the reversibly soluble matrix eudragit l-100 by non-covalent and covalent methods. covalent immobilization using carbodiimide coupling exhibited superior enzyme loading and reusability compared with non-covalent immobilization, and the covalent loading was increased by almost 20% through the addition of n-hydroxysuccinimide. the temperature optimum of the cellulase was not improved apparently by immobilization but the ph optimum increased from 4.75 to 5.25. immobilized cellulase was more active than free cellulase above ph 5.0. immobilized cellulase was more stable than free cellulase during storage at 4 degrees c, room temperature and 50 degrees c. k(m) values of immobilized and free cellulase were 85.55 and 73.84 g l(-1), respectively. about 50% productivity was retained after five cycles for hydrolysis of steam-exploded straw. |
| 英文摘要 | to prepare a smart biocatalyst, cellulase was immobilized on the reversibly soluble matrix eudragit l-100 by non-covalent and covalent methods. covalent immobilization using carbodiimide coupling exhibited superior enzyme loading and reusability compared with non-covalent immobilization, and the covalent loading was increased by almost 20% through the addition of n-hydroxysuccinimide. the temperature optimum of the cellulase was not improved apparently by immobilization but the ph optimum increased from 4.75 to 5.25. immobilized cellulase was more active than free cellulase above ph 5.0. immobilized cellulase was more stable than free cellulase during storage at 4 degrees c, room temperature and 50 degrees c. k(m) values of immobilized and free cellulase were 85.55 and 73.84 g l(-1), respectively. about 50% productivity was retained after five cycles for hydrolysis of steam-exploded straw. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | biochemistry & molecular biology ; biotechnology & applied microbiology |
| 类目[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology |
| 研究领域[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology |
| 关键词[WOS] | xylan-degrading enzymes ; bioethanol production ; enzymatic-hydrolysis ; polymer ; ethanol ; fermentation ; pretreatment ; carrageenan ; improvement ; chitosan |
| 收录类别 | SCI |
| 资助信息 | chinese academy of sciences [kscx-yw-11-a3, kscx2-yw-g-075-09, kgcx2-yw-335]; national high technology research and development program of china [2007aa05z406] |
| 原文出处 | https://dx.doi.org/10.3109/10242422.2010.516391 |
| 语种 | 英语 |
| WOS记录号 | WOS:000285554000003 |
| 公开日期 | 2014-12-24 |
| 源URL | [http://ir.giec.ac.cn/handle/344007/8462]  |
| 专题 | 中国科学院广州能源研究所 生物质能源生化转化实验室 |
| 作者单位 | 1.Chinese Acad Sci, Guangzhou Inst Energy & Convers, Key Lab Renewable Energy & Gas Hydrate, Guangzhou 510640, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yu,Xu, Jing-Liang,Li, Dong,et al. Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100[J]. biocatalysis and biotransformation,2010,28(5-6):313-319. |
| APA | Zhang, Yu,Xu, Jing-Liang,Li, Dong,&Yuan, Zhen-Hong.(2010).Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100.biocatalysis and biotransformation,28(5-6),313-319. |
| MLA | Zhang, Yu,et al."Preparation and properties of an immobilized cellulase on the reversibly soluble matrix Eudragit L-100".biocatalysis and biotransformation 28.5-6(2010):313-319. |
入库方式： OAI收割
来源：广州能源研究所
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