中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.)

文献类型:期刊论文

作者Lei, Yunting1; Zhao, Yanyan1,2; Yu, Feng1,2; Li, Yuan1,2; Dou, Quanwen1
刊名conservation genetics resources
出版日期2014-12-01
卷号6期号:4页码:861-864
关键词Siberian wildrye Elymus sibiricus Genomic-SSR Polymorphism
ISSN号1877-7252
中文摘要siberian wildrye, elymus sibiricus l., is an important perennial forage crop in temperate regions of the world, especially in the qinghai-tibet plateau. however, it is now threatened by climate warming, excessive grazing, and damage to its natural habitat. a total of 53 polymorphic genomic-ssr markers were isolated and characterized in e. sibiricus. the number of alleles for these markers in 11 individuals varied from 2 to 5, with an average of 3.09 alleles per locus. segregation analysis in an f-2 population revealed that the tested genomic-ssr markers had a co-dominant nature and mendelian inheritance. cross-species amplification tests showed that about half of the total e. sibiricus genomic-ssr markers could be effectively amplified in e. nutans. these ssr markers are the first characterized in e. sibiricus and will be useful for investigating genetic diversity and molecular-assisted breeding.
英文摘要siberian wildrye, elymus sibiricus l., is an important perennial forage crop in temperate regions of the world, especially in the qinghai-tibet plateau. however, it is now threatened by climate warming, excessive grazing, and damage to its natural habitat. a total of 53 polymorphic genomic-ssr markers were isolated and characterized in e. sibiricus. the number of alleles for these markers in 11 individuals varied from 2 to 5, with an average of 3.09 alleles per locus. segregation analysis in an f-2 population revealed that the tested genomic-ssr markers had a co-dominant nature and mendelian inheritance. cross-species amplification tests showed that about half of the total e. sibiricus genomic-ssr markers could be effectively amplified in e. nutans. these ssr markers are the first characterized in e. sibiricus and will be useful for investigating genetic diversity and molecular-assisted breeding.
WOS标题词science & technology ; life sciences & biomedicine
类目[WOS]biodiversity conservation ; genetics & heredity
研究领域[WOS]biodiversity & conservation ; genetics & heredity
收录类别SCI
语种英语
WOS记录号WOS:000344481900014
公开日期2014-12-19
源URL[http://ir.nwipb.ac.cn/handle/363003/4172]  
专题西北高原生物研究所_中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Lei, Yunting,Zhao, Yanyan,Yu, Feng,et al. Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.)[J]. conservation genetics resources,2014,6(4):861-864.
APA Lei, Yunting,Zhao, Yanyan,Yu, Feng,Li, Yuan,&Dou, Quanwen.(2014).Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.).conservation genetics resources,6(4),861-864.
MLA Lei, Yunting,et al."Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.)".conservation genetics resources 6.4(2014):861-864.

入库方式: OAI收割

来源:西北高原生物研究所

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