Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.)
文献类型:期刊论文
作者 | Lei, Yunting1; Zhao, Yanyan1,2; Yu, Feng1,2; Li, Yuan1,2; Dou, Quanwen1 |
刊名 | conservation genetics resources
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出版日期 | 2014-12-01 |
卷号 | 6期号:4页码:861-864 |
关键词 | Siberian wildrye Elymus sibiricus Genomic-SSR Polymorphism |
ISSN号 | 1877-7252 |
中文摘要 | siberian wildrye, elymus sibiricus l., is an important perennial forage crop in temperate regions of the world, especially in the qinghai-tibet plateau. however, it is now threatened by climate warming, excessive grazing, and damage to its natural habitat. a total of 53 polymorphic genomic-ssr markers were isolated and characterized in e. sibiricus. the number of alleles for these markers in 11 individuals varied from 2 to 5, with an average of 3.09 alleles per locus. segregation analysis in an f-2 population revealed that the tested genomic-ssr markers had a co-dominant nature and mendelian inheritance. cross-species amplification tests showed that about half of the total e. sibiricus genomic-ssr markers could be effectively amplified in e. nutans. these ssr markers are the first characterized in e. sibiricus and will be useful for investigating genetic diversity and molecular-assisted breeding. |
英文摘要 | siberian wildrye, elymus sibiricus l., is an important perennial forage crop in temperate regions of the world, especially in the qinghai-tibet plateau. however, it is now threatened by climate warming, excessive grazing, and damage to its natural habitat. a total of 53 polymorphic genomic-ssr markers were isolated and characterized in e. sibiricus. the number of alleles for these markers in 11 individuals varied from 2 to 5, with an average of 3.09 alleles per locus. segregation analysis in an f-2 population revealed that the tested genomic-ssr markers had a co-dominant nature and mendelian inheritance. cross-species amplification tests showed that about half of the total e. sibiricus genomic-ssr markers could be effectively amplified in e. nutans. these ssr markers are the first characterized in e. sibiricus and will be useful for investigating genetic diversity and molecular-assisted breeding. |
WOS标题词 | science & technology ; life sciences & biomedicine |
类目[WOS] | biodiversity conservation ; genetics & heredity |
研究领域[WOS] | biodiversity & conservation ; genetics & heredity |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000344481900014 |
公开日期 | 2014-12-19 |
源URL | [http://ir.nwipb.ac.cn/handle/363003/4172] ![]() |
专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
作者单位 | 1.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Lei, Yunting,Zhao, Yanyan,Yu, Feng,et al. Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.)[J]. conservation genetics resources,2014,6(4):861-864. |
APA | Lei, Yunting,Zhao, Yanyan,Yu, Feng,Li, Yuan,&Dou, Quanwen.(2014).Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.).conservation genetics resources,6(4),861-864. |
MLA | Lei, Yunting,et al."Development and characterization of 53 polymorphic genomic-SSR markers in Siberian wildrye (Elymus sibiricus L.)".conservation genetics resources 6.4(2014):861-864. |
入库方式: OAI收割
来源:西北高原生物研究所
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