中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Sensitive Determination of Panaxadiol Using Rhodamine B as Sensitizing Derivatization Reagent by Ultrahigh Performance Liquid Chromatography Triple Quadrupole Mass Spectrometry

文献类型:期刊论文

作者Zhao Xian-En1; Lu Tao1; Wei Na1; Zhu Shu-Yun1; You Jin-Mao1,2
刊名chinese journal of analytical chemistry
出版日期2014-11-01
卷号42期号:11页码:1629-1633
关键词Ultrasonic-assisted dispersive liquid-liquid microextraction Rhodamine B Derivatization Mass spectrometry sensitization Panaxadiol
ISSN号0253-3820
中文摘要commercial rhodamine b was first reported as a derivatization reagent labeling hydroxyl compound for mass spectrometry sensitization. in virtue of the strategy of ultrasonic-assisted dispersive liquid-liquid microextraction (ua-dllme) combined with rhodamine b derivatization, a new method of ultrahigh performance liquid chromatography triple quadrupole mass spectrometry (uhplc-ms/ms) was developed for the sensitive determination of panaxadiol (pd). ua-dllme and derivatization conditions were investigated. pd in cosmetics and ginseng was extracted by ua-dllme (150 mu l chloroform as extraction solvent, 500 mu l ethanol as disperser solvent, 3 min). using rhodamine b as precolumn derivatization reagent, the stable derivative of pd was obtained in acetonitrile at 70 degrees c for 30 min under microwave radiation (800 w) with the catalyst of n, n'-carbonyldiimidazole (cdi) and 4-dimethylaminopyridine (dmap). and then the pd derivative was separated and detected within 3 min by multiple reaction monitoring (mrm) mode of uhplc-ms/ms. the lod was 4.0 ng l-1, and the loq was 15.0 ng l-1. this method had perfect linearity, precision and recovery results, and showed obvious advantages such as sensitivity and low matrix effect in comparison with the previously reported methods.
英文摘要commercial rhodamine b was first reported as a derivatization reagent labeling hydroxyl compound for mass spectrometry sensitization. in virtue of the strategy of ultrasonic-assisted dispersive liquid-liquid microextraction (ua-dllme) combined with rhodamine b derivatization, a new method of ultrahigh performance liquid chromatography triple quadrupole mass spectrometry (uhplc-ms/ms) was developed for the sensitive determination of panaxadiol (pd). ua-dllme and derivatization conditions were investigated. pd in cosmetics and ginseng was extracted by ua-dllme (150 mu l chloroform as extraction solvent, 500 mu l ethanol as disperser solvent, 3 min). using rhodamine b as precolumn derivatization reagent, the stable derivative of pd was obtained in acetonitrile at 70 degrees c for 30 min under microwave radiation (800 w) with the catalyst of n, n'-carbonyldiimidazole (cdi) and 4-dimethylaminopyridine (dmap). and then the pd derivative was separated and detected within 3 min by multiple reaction monitoring (mrm) mode of uhplc-ms/ms. the lod was 4.0 ng l-1, and the loq was 15.0 ng l-1. this method had perfect linearity, precision and recovery results, and showed obvious advantages such as sensitivity and low matrix effect in comparison with the previously reported methods.
WOS标题词science & technology ; physical sciences
类目[WOS]chemistry, analytical
研究领域[WOS]chemistry
关键词[WOS]fluorescence ; ginsenosides
收录类别SCI
语种英语
WOS记录号WOS:000345116900006
公开日期2014-12-19
源URL[http://ir.nwipb.ac.cn/handle/363003/4175]  
专题西北高原生物研究所_中国科学院西北高原生物研究所
作者单位1.Qufu Normal Univ, Coll Chem & Chem Engn, Shandong Prov Key Lab Life Organ Anal, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China
推荐引用方式
GB/T 7714
Zhao Xian-En,Lu Tao,Wei Na,et al. Sensitive Determination of Panaxadiol Using Rhodamine B as Sensitizing Derivatization Reagent by Ultrahigh Performance Liquid Chromatography Triple Quadrupole Mass Spectrometry[J]. chinese journal of analytical chemistry,2014,42(11):1629-1633.
APA Zhao Xian-En,Lu Tao,Wei Na,Zhu Shu-Yun,&You Jin-Mao.(2014).Sensitive Determination of Panaxadiol Using Rhodamine B as Sensitizing Derivatization Reagent by Ultrahigh Performance Liquid Chromatography Triple Quadrupole Mass Spectrometry.chinese journal of analytical chemistry,42(11),1629-1633.
MLA Zhao Xian-En,et al."Sensitive Determination of Panaxadiol Using Rhodamine B as Sensitizing Derivatization Reagent by Ultrahigh Performance Liquid Chromatography Triple Quadrupole Mass Spectrometry".chinese journal of analytical chemistry 42.11(2014):1629-1633.

入库方式: OAI收割

来源:西北高原生物研究所

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