Sensitive Determination of Panaxadiol Using Rhodamine B as Sensitizing Derivatization Reagent by Ultrahigh Performance Liquid Chromatography Triple Quadrupole Mass Spectrometry
文献类型：期刊论文
| 作者 | Zhao Xian-En1; Lu Tao1; Wei Na1; Zhu Shu-Yun1; You Jin-Mao1,2 |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2014-11-01 |
| 卷号 | 42期号:11页码:1629-1633 |
| 关键词 | Ultrasonic-assisted dispersive liquid-liquid microextraction Rhodamine B Derivatization Mass spectrometry sensitization Panaxadiol |
| ISSN号 | 0253-3820 |
| 中文摘要 | commercial rhodamine b was first reported as a derivatization reagent labeling hydroxyl compound for mass spectrometry sensitization. in virtue of the strategy of ultrasonic-assisted dispersive liquid-liquid microextraction (ua-dllme) combined with rhodamine b derivatization, a new method of ultrahigh performance liquid chromatography triple quadrupole mass spectrometry (uhplc-ms/ms) was developed for the sensitive determination of panaxadiol (pd). ua-dllme and derivatization conditions were investigated. pd in cosmetics and ginseng was extracted by ua-dllme (150 mu l chloroform as extraction solvent, 500 mu l ethanol as disperser solvent, 3 min). using rhodamine b as precolumn derivatization reagent, the stable derivative of pd was obtained in acetonitrile at 70 degrees c for 30 min under microwave radiation (800 w) with the catalyst of n, n'-carbonyldiimidazole (cdi) and 4-dimethylaminopyridine (dmap). and then the pd derivative was separated and detected within 3 min by multiple reaction monitoring (mrm) mode of uhplc-ms/ms. the lod was 4.0 ng l-1, and the loq was 15.0 ng l-1. this method had perfect linearity, precision and recovery results, and showed obvious advantages such as sensitivity and low matrix effect in comparison with the previously reported methods. |
| 英文摘要 | commercial rhodamine b was first reported as a derivatization reagent labeling hydroxyl compound for mass spectrometry sensitization. in virtue of the strategy of ultrasonic-assisted dispersive liquid-liquid microextraction (ua-dllme) combined with rhodamine b derivatization, a new method of ultrahigh performance liquid chromatography triple quadrupole mass spectrometry (uhplc-ms/ms) was developed for the sensitive determination of panaxadiol (pd). ua-dllme and derivatization conditions were investigated. pd in cosmetics and ginseng was extracted by ua-dllme (150 mu l chloroform as extraction solvent, 500 mu l ethanol as disperser solvent, 3 min). using rhodamine b as precolumn derivatization reagent, the stable derivative of pd was obtained in acetonitrile at 70 degrees c for 30 min under microwave radiation (800 w) with the catalyst of n, n'-carbonyldiimidazole (cdi) and 4-dimethylaminopyridine (dmap). and then the pd derivative was separated and detected within 3 min by multiple reaction monitoring (mrm) mode of uhplc-ms/ms. the lod was 4.0 ng l-1, and the loq was 15.0 ng l-1. this method had perfect linearity, precision and recovery results, and showed obvious advantages such as sensitivity and low matrix effect in comparison with the previously reported methods. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | fluorescence ; ginsenosides |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000345116900006 |
| 公开日期 | 2014-12-19 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/4175]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Coll Chem & Chem Engn, Shandong Prov Key Lab Life Organ Anal, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao Xian-En,Lu Tao,Wei Na,et al. Sensitive Determination of Panaxadiol Using Rhodamine B as Sensitizing Derivatization Reagent by Ultrahigh Performance Liquid Chromatography Triple Quadrupole Mass Spectrometry[J]. chinese journal of analytical chemistry,2014,42(11):1629-1633. |
| APA | Zhao Xian-En,Lu Tao,Wei Na,Zhu Shu-Yun,&You Jin-Mao.(2014).Sensitive Determination of Panaxadiol Using Rhodamine B as Sensitizing Derivatization Reagent by Ultrahigh Performance Liquid Chromatography Triple Quadrupole Mass Spectrometry.chinese journal of analytical chemistry,42(11),1629-1633. |
| MLA | Zhao Xian-En,et al."Sensitive Determination of Panaxadiol Using Rhodamine B as Sensitizing Derivatization Reagent by Ultrahigh Performance Liquid Chromatography Triple Quadrupole Mass Spectrometry".chinese journal of analytical chemistry 42.11(2014):1629-1633. |
入库方式： OAI收割
来源：西北高原生物研究所
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