Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells
文献类型：期刊论文
| 作者 | Wang, Yi1; Nie, Fangyuan1; Ouyang, Jian2; Wang, Xiaoyan2; Ma, Xiaofeng1 |
| 刊名 | tumor biology
 |
| 出版日期 | 2014-10-01 |
| 卷号 | 35期号:10页码:9563-9569 |
| 关键词 | Fatty acid synthase Inhibitor Sea buckthorn Procyanidins MDA-MB-231 cells Cell apoptosis |
| ISSN号 | 1010-4283 |
| 中文摘要 | fatty acid synthase (fas) is overexpressed in many human cancers including breast cancer and is considered to be a promising target for therapy. sea buckthorn has long been used to treat a variety of maladies. here, we investigated the inhibitory effect of sea buckthorn procyanidins (sbps) isolated from the seeds of sea buckthorn on fas and fas overexpressed human breast cancer mda-mb-231 cells. the fas activity and fas inhibition were measured by a spectrophotometer at 340 nm of nicotinamide adenine dinucleotide phosphate (nadph) absorption. we found that sbp potently inhibited the activity of fas with a half-inhibitory concentration (ic50) value of 0.087 mu g/ml. 3-4,5-dimethylthiazol- 2-yl-2,3-diphenyl tetrazolium bromide (mtt) assay was used to test the cell viability. sbp reduced mda-mb-231 cell viability with an ic50 value of 37.5 mu g/ml. hoechst 33258/propidium iodide dual staining and flow cytometric analysis showed that sbp induced mda-mb-231 cell apoptosis. sbp inhibited intracellular fas activity with a dose-dependent manner. in addition, sodium palmitate could rescue the cell apoptosis induced by sbp. these results showed that sbp was a promising fas inhibitor which could induce the apoptosis of mda-mb-231 cells via inhibiting fas. these findings suggested that sbp might be useful for preventing or treating breast cancer. |
| 英文摘要 | fatty acid synthase (fas) is overexpressed in many human cancers including breast cancer and is considered to be a promising target for therapy. sea buckthorn has long been used to treat a variety of maladies. here, we investigated the inhibitory effect of sea buckthorn procyanidins (sbps) isolated from the seeds of sea buckthorn on fas and fas overexpressed human breast cancer mda-mb-231 cells. the fas activity and fas inhibition were measured by a spectrophotometer at 340 nm of nicotinamide adenine dinucleotide phosphate (nadph) absorption. we found that sbp potently inhibited the activity of fas with a half-inhibitory concentration (ic50) value of 0.087 mu g/ml. 3-4,5-dimethylthiazol- 2-yl-2,3-diphenyl tetrazolium bromide (mtt) assay was used to test the cell viability. sbp reduced mda-mb-231 cell viability with an ic50 value of 37.5 mu g/ml. hoechst 33258/propidium iodide dual staining and flow cytometric analysis showed that sbp induced mda-mb-231 cell apoptosis. sbp inhibited intracellular fas activity with a dose-dependent manner. in addition, sodium palmitate could rescue the cell apoptosis induced by sbp. these results showed that sbp was a promising fas inhibitor which could induce the apoptosis of mda-mb-231 cells via inhibiting fas. these findings suggested that sbp might be useful for preventing or treating breast cancer. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | oncology |
| 研究领域[WOS] | oncology |
| 关键词[WOS] | seabuckthorn hippophae-rhamnoides ; induced oxidative stress ; nf-kappa-b ; cancer-cells ; epigallocatechin gallate ; antitumor-activity ; molecular targets ; natural inhibitor ; prostate-cancer ; palmitic acid |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000344532700014 |
| 公开日期 | 2014-12-19 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/4181]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Qinghai Provinc, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yi,Nie, Fangyuan,Ouyang, Jian,et al. Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells[J]. tumor biology,2014,35(10):9563-9569. |
| APA | Wang, Yi,Nie, Fangyuan,Ouyang, Jian,Wang, Xiaoyan,&Ma, Xiaofeng.(2014).Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells.tumor biology,35(10),9563-9569. |
| MLA | Wang, Yi,et al."Inhibitory effects of sea buckthorn procyanidins on fatty acid synthase and MDA-MB-231 cells".tumor biology 35.10(2014):9563-9569. |
入库方式： OAI收割
来源：西北高原生物研究所
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