The detection of a de novo allele of the Glu-1Dx gene in wheat-rye hybrid offspring
文献类型：期刊论文
| 作者 | Yuan, Zhongwei1; Liu, Miao1; Ouyang, Yuyuan1; Zeng, Xiaoxue1; Hao, Ming1; Zhang, Lianquan1; Ning, Shunzong1; Yan, Zehong1; Liu, Dengcai1,2 |
| 刊名 | theoretical and applied genetics
 |
| 出版日期 | 2014-10-01 |
| 卷号 | 127期号:10页码:2173-2182 |
| ISSN号 | 0040-5752 |
| 中文摘要 | this study provides a link between a de novo gene and novel phenotype in wheat-rye hybrids that can be used as a model for induced de novo genetic variation. wide hybridization can produce de novo dna variation that may cause novel phenotypes. however, there is still a lack of specific links between changed genes and novel phenotypes in wide hybrids. the well-studied high-molecular-weight glutenin subunit (hmw-gs) genes in tribe triticeae provide a useful model for addressing this issue. in this study, we investigated the feasibility of a wheat-rye hybridization method for inducing de novo phenotypes using the glu-1dx2.2 subunit as an example. we developed three hexaploid wheat lines with normal fertility and a glu-1dx2.2 variant, named glu-1dx2.2 (v) , derived from three f-1 hybrids. the wild-type glu-1dx2.2 has two direct repeats of 295 bp length separated by an intervening 101 bp in its central repetitive region. in the mutant glu-1dx2.2 (v) , one copy of the repeats and the intervening sequence were deleted, probably through homology-dependent illegitimate recombination (ir). this study provides a direct link between a de novo allele and novel phenotype. our results indicate that the wheat-rye method may be a useful tool to induce de novo genetic variations that broaden the genetic diversity for wheat improvement. |
| 英文摘要 | this study provides a link between a de novo gene and novel phenotype in wheat-rye hybrids that can be used as a model for induced de novo genetic variation. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | agronomy ; plant sciences ; genetics & heredity ; horticulture |
| 研究领域[WOS] | agriculture ; plant sciences ; genetics & heredity |
| 关键词[WOS] | high-molecular-weight ; synthetic hexaploid wheat ; hmw glutenin subunits ; genome evolution ; illegitimate recombination ; allopolyploid wheat ; wide hybridization ; tetraploid wheat ; crop improvement ; common wheat |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000342494500010 |
| 公开日期 | 2014-12-19 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/4189]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Sichuan Agr Univ, Triticeae Res Inst, Wenjiang 611130, Sichuan, Peoples R China 2.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Northwest Inst Plateau Biol, Xining 810001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yuan, Zhongwei,Liu, Miao,Ouyang, Yuyuan,et al. The detection of a de novo allele of the Glu-1Dx gene in wheat-rye hybrid offspring[J]. theoretical and applied genetics,2014,127(10):2173-2182. |
| APA | Yuan, Zhongwei.,Liu, Miao.,Ouyang, Yuyuan.,Zeng, Xiaoxue.,Hao, Ming.,...&Liu, Dengcai.(2014).The detection of a de novo allele of the Glu-1Dx gene in wheat-rye hybrid offspring.theoretical and applied genetics,127(10),2173-2182. |
| MLA | Yuan, Zhongwei,et al."The detection of a de novo allele of the Glu-1Dx gene in wheat-rye hybrid offspring".theoretical and applied genetics 127.10(2014):2173-2182. |
入库方式： OAI收割
来源：西北高原生物研究所
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