DETERMINATION OF FREE FATTY ACIDS IN CORTEX LYCII BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY WITH FLUORESCENCE DETECTION AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION IDENTIFICATION
文献类型：期刊论文
| 作者 | Chen, Xiangming1,2,3; Dong, Xiuli2; Suo, Yourui1; You, Jinmao1,4 |
| 刊名 | journal of liquid chromatography & related technologies
 |
| 出版日期 | 2014-07-03 |
| 卷号 | 37期号:11页码:1548-1557 |
| 关键词 | Cortex Lycii pre-column derivatization fatty acids HPLC/MS fluorescence detection |
| ISSN号 | 1082-6076 |
| 中文摘要 | a highly sensitive method for the determination of free fatty acids (ffas) using high-performance liquid chromatography (hplc) with fluorescence detection has been developed. twenty-six ffas (c5-c30) were derivatized by a novel pre-column derivatization reagent 2-(11h-benzo[a]carbazol-11-yl)-ethyl-4-methyl- benzenesulphonate (bcets). derivatives were sufficiently stable to be efficiently analyzed by hplc. all fatty acid derivatives were separated on a hypersil bds-c8 column in conjunction with a gradient elution with a good baseline resolution. the identification was carried out by post-column online mass spectrometry with atmospheric pressure chemical ionization (apci) in positive-ion detection mode. the established method exhibited good selectivity and excellent repeatability. the rsd (n=6) for each fatty acid derivative was <2.8%, and detection limit (at signal-to-noise of 3:1) was 15-64 fmol. the method was used to quantify ffas in cortex lycii sample with satisfactory results. |
| 英文摘要 | a highly sensitive method for the determination of free fatty acids (ffas) using high-performance liquid chromatography (hplc) with fluorescence detection has been developed. twenty-six ffas (c5-c30) were derivatized by a novel pre-column derivatization reagent 2-(11h-benzo[a]carbazol-11-yl)-ethyl-4-methyl- benzenesulphonate (bcets). derivatives were sufficiently stable to be efficiently analyzed by hplc. all fatty acid derivatives were separated on a hypersil bds-c8 column in conjunction with a gradient elution with a good baseline resolution. the identification was carried out by post-column online mass spectrometry with atmospheric pressure chemical ionization (apci) in positive-ion detection mode. the established method exhibited good selectivity and excellent repeatability. the rsd (n=6) for each fatty acid derivative was <2.8%, and detection limit (at signal-to-noise of 3:1) was 15-64 fmol. the method was used to quantify ffas in cortex lycii sample with satisfactory results. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | carboxylic-acids ; derivatization reagent ; labeling reagent ; fluorometric detection ; gas-chromatography ; biological samples ; hplc ; 9-anthryldiazomethane ; ms ; metabolites |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000333991500005 |
| 公开日期 | 2014-12-19 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/4210]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Plateau Inst Biol, Xining 810001, Qinghai, Peoples R China 2.Binzhou Med Univ, Coll Pharm, Yantai, Shandong, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 4.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal, Qufu, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Xiangming,Dong, Xiuli,Suo, Yourui,et al. DETERMINATION OF FREE FATTY ACIDS IN CORTEX LYCII BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY WITH FLUORESCENCE DETECTION AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION IDENTIFICATION[J]. journal of liquid chromatography & related technologies,2014,37(11):1548-1557. |
| APA | Chen, Xiangming,Dong, Xiuli,Suo, Yourui,&You, Jinmao.(2014).DETERMINATION OF FREE FATTY ACIDS IN CORTEX LYCII BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY WITH FLUORESCENCE DETECTION AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION IDENTIFICATION.journal of liquid chromatography & related technologies,37(11),1548-1557. |
| MLA | Chen, Xiangming,et al."DETERMINATION OF FREE FATTY ACIDS IN CORTEX LYCII BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY MASS SPECTROMETRY WITH FLUORESCENCE DETECTION AND ATMOSPHERIC PRESSURE CHEMICAL IONIZATION IDENTIFICATION".journal of liquid chromatography & related technologies 37.11(2014):1548-1557. |
入库方式： OAI收割
来源：西北高原生物研究所
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