中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Purification and Characterization of Aporphine Alkaloids from Leaves of Nelumbo nucifera Gaertn and Their Effects on Glucose Consumption in 3T3-L1 Adipocytes

文献类型:期刊论文

作者Ma, Chengjun1; Wang, Jinjun1; Chu, Hongmei2; Zhang, Xiaoxiao1; Wang, Zhenhua1; Wang, Honglun3; Li, Gang1,3
刊名international journal of molecular sciences
出版日期2014-03-01
卷号15期号:3页码:3481-3494
关键词Nelumbo nucifera Gaertn aporphine alkaloids high-speed counter-current chromatography (HSCCC) isolation 3T3-L1 adipocytes glucose consumption
ISSN号1422-0067
中文摘要aporphine alkaloids from the leaves of nelumbo nucifera gaertn are substances of great interest because of their important pharmacological activities, particularly anti-diabetic, anti-obesity, anti-hyperlipidemic, anti-oxidant, and anti-hiv's activities. in order to produce large amounts of pure alkaloid for research purposes, a novel method using high-speed counter-current chromatography (hsccc) was developed. without any initial cleanup steps, four main aporphine alkaloids, including 2-hydroxy-1-methoxyaporphine, pronuciferine, nuciferine and roemerine were successfully purified from the crude extract by hsccc in one step. the separation was performed with a simple two-phase solvent system composed of n-hexane-ethyl acetate-methanol-acetonitrile-water (5:3:3:2.5:5, v/v/v/v/v). in each operation, 100 mg crude extracts was separated and yielded 6.3 mg of 2-hydroxy-1-methoxyaporphine (95.1% purity), 1.1 mg of pronuciferine (96.8% purity), 8.5 mg of nuciferine (98.9% purity), and 2.7 mg of roemerine (97.4%) respectively. the chemical structure of four aporphine alkaloids are identified by means of electrospray ionization ms (esi-ms) and nuclear magnetic resonance (nmr) analysis. moreover, the effects of four separated aporphine alkaloids on insulin-stimulated glucose consumption were examined in 3t3-l1 adipocytes. the results showed that 2-hydroxy-1-methoxyaporphine and pronuciferine increased the glucose consumption significantly as rosiglitazone did.
英文摘要aporphine alkaloids from the leaves of nelumbo nucifera gaertn are substances of great interest because of their important pharmacological activities, particularly anti-diabetic, anti-obesity, anti-hyperlipidemic, anti-oxidant, and anti-hiv's activities. in order to produce large amounts of pure alkaloid for research purposes, a novel method using high-speed counter-current chromatography (hsccc) was developed. without any initial cleanup steps, four main aporphine alkaloids, including 2-hydroxy-1-methoxyaporphine, pronuciferine, nuciferine and roemerine were successfully purified from the crude extract by hsccc in one step. the separation was performed with a simple two-phase solvent system composed of n-hexane-ethyl acetate-methanol-acetonitrile-water (5:3:3:2.5:5, v/v/v/v/v). in each operation, 100 mg crude extracts was separated and yielded 6.3 mg of 2-hydroxy-1-methoxyaporphine (95.1% purity), 1.1 mg of pronuciferine (96.8% purity), 8.5 mg of nuciferine (98.9% purity), and 2.7 mg of roemerine (97.4%) respectively. the chemical structure of four aporphine alkaloids are identified by means of electrospray ionization ms (esi-ms) and nuclear magnetic resonance (nmr) analysis. moreover, the effects of four separated aporphine alkaloids on insulin-stimulated glucose consumption were examined in 3t3-l1 adipocytes. the results showed that 2-hydroxy-1-methoxyaporphine and pronuciferine increased the glucose consumption significantly as rosiglitazone did.
WOS标题词science & technology ; physical sciences
类目[WOS]chemistry, multidisciplinary
研究领域[WOS]chemistry
关键词[WOS]counter-current chromatography ; constituents ; boldine ; cells ; mice ; benzylisoquinoline ; adiponectin ; metabolites ; extract ; rats
收录类别SCI
语种英语
WOS记录号WOS:000334444700006
公开日期2014-12-19
源URL[http://ir.nwipb.ac.cn/handle/363003/4246]  
专题西北高原生物研究所_中国科学院西北高原生物研究所
作者单位1.Yantai Univ, Sch Life Sci, Yantai 264005, Peoples R China
2.Yantai Univ, Sch Pharm, Yantai 264005, Peoples R China
3.Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, Xining 810001, Peoples R China
推荐引用方式
GB/T 7714
Ma, Chengjun,Wang, Jinjun,Chu, Hongmei,et al. Purification and Characterization of Aporphine Alkaloids from Leaves of Nelumbo nucifera Gaertn and Their Effects on Glucose Consumption in 3T3-L1 Adipocytes[J]. international journal of molecular sciences,2014,15(3):3481-3494.
APA Ma, Chengjun.,Wang, Jinjun.,Chu, Hongmei.,Zhang, Xiaoxiao.,Wang, Zhenhua.,...&Li, Gang.(2014).Purification and Characterization of Aporphine Alkaloids from Leaves of Nelumbo nucifera Gaertn and Their Effects on Glucose Consumption in 3T3-L1 Adipocytes.international journal of molecular sciences,15(3),3481-3494.
MLA Ma, Chengjun,et al."Purification and Characterization of Aporphine Alkaloids from Leaves of Nelumbo nucifera Gaertn and Their Effects on Glucose Consumption in 3T3-L1 Adipocytes".international journal of molecular sciences 15.3(2014):3481-3494.

入库方式: OAI收割

来源:西北高原生物研究所

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