Purification and Characterization of Aporphine Alkaloids from Leaves of Nelumbo nucifera Gaertn and Their Effects on Glucose Consumption in 3T3-L1 Adipocytes
文献类型：期刊论文
| 作者 | Ma, Chengjun1; Wang, Jinjun1; Chu, Hongmei2; Zhang, Xiaoxiao1; Wang, Zhenhua1; Wang, Honglun3; Li, Gang1,3 |
| 刊名 | international journal of molecular sciences
 |
| 出版日期 | 2014-03-01 |
| 卷号 | 15期号:3页码:3481-3494 |
| 关键词 | Nelumbo nucifera Gaertn aporphine alkaloids high-speed counter-current chromatography (HSCCC) isolation 3T3-L1 adipocytes glucose consumption |
| ISSN号 | 1422-0067 |
| 中文摘要 | aporphine alkaloids from the leaves of nelumbo nucifera gaertn are substances of great interest because of their important pharmacological activities, particularly anti-diabetic, anti-obesity, anti-hyperlipidemic, anti-oxidant, and anti-hiv's activities. in order to produce large amounts of pure alkaloid for research purposes, a novel method using high-speed counter-current chromatography (hsccc) was developed. without any initial cleanup steps, four main aporphine alkaloids, including 2-hydroxy-1-methoxyaporphine, pronuciferine, nuciferine and roemerine were successfully purified from the crude extract by hsccc in one step. the separation was performed with a simple two-phase solvent system composed of n-hexane-ethyl acetate-methanol-acetonitrile-water (5:3:3:2.5:5, v/v/v/v/v). in each operation, 100 mg crude extracts was separated and yielded 6.3 mg of 2-hydroxy-1-methoxyaporphine (95.1% purity), 1.1 mg of pronuciferine (96.8% purity), 8.5 mg of nuciferine (98.9% purity), and 2.7 mg of roemerine (97.4%) respectively. the chemical structure of four aporphine alkaloids are identified by means of electrospray ionization ms (esi-ms) and nuclear magnetic resonance (nmr) analysis. moreover, the effects of four separated aporphine alkaloids on insulin-stimulated glucose consumption were examined in 3t3-l1 adipocytes. the results showed that 2-hydroxy-1-methoxyaporphine and pronuciferine increased the glucose consumption significantly as rosiglitazone did. |
| 英文摘要 | aporphine alkaloids from the leaves of nelumbo nucifera gaertn are substances of great interest because of their important pharmacological activities, particularly anti-diabetic, anti-obesity, anti-hyperlipidemic, anti-oxidant, and anti-hiv's activities. in order to produce large amounts of pure alkaloid for research purposes, a novel method using high-speed counter-current chromatography (hsccc) was developed. without any initial cleanup steps, four main aporphine alkaloids, including 2-hydroxy-1-methoxyaporphine, pronuciferine, nuciferine and roemerine were successfully purified from the crude extract by hsccc in one step. the separation was performed with a simple two-phase solvent system composed of n-hexane-ethyl acetate-methanol-acetonitrile-water (5:3:3:2.5:5, v/v/v/v/v). in each operation, 100 mg crude extracts was separated and yielded 6.3 mg of 2-hydroxy-1-methoxyaporphine (95.1% purity), 1.1 mg of pronuciferine (96.8% purity), 8.5 mg of nuciferine (98.9% purity), and 2.7 mg of roemerine (97.4%) respectively. the chemical structure of four aporphine alkaloids are identified by means of electrospray ionization ms (esi-ms) and nuclear magnetic resonance (nmr) analysis. moreover, the effects of four separated aporphine alkaloids on insulin-stimulated glucose consumption were examined in 3t3-l1 adipocytes. the results showed that 2-hydroxy-1-methoxyaporphine and pronuciferine increased the glucose consumption significantly as rosiglitazone did. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | counter-current chromatography ; constituents ; boldine ; cells ; mice ; benzylisoquinoline ; adiponectin ; metabolites ; extract ; rats |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000334444700006 |
| 公开日期 | 2014-12-19 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/4246]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Yantai Univ, Sch Life Sci, Yantai 264005, Peoples R China 2.Yantai Univ, Sch Pharm, Yantai 264005, Peoples R China 3.Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, Xining 810001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Chengjun,Wang, Jinjun,Chu, Hongmei,et al. Purification and Characterization of Aporphine Alkaloids from Leaves of Nelumbo nucifera Gaertn and Their Effects on Glucose Consumption in 3T3-L1 Adipocytes[J]. international journal of molecular sciences,2014,15(3):3481-3494. |
| APA | Ma, Chengjun.,Wang, Jinjun.,Chu, Hongmei.,Zhang, Xiaoxiao.,Wang, Zhenhua.,...&Li, Gang.(2014).Purification and Characterization of Aporphine Alkaloids from Leaves of Nelumbo nucifera Gaertn and Their Effects on Glucose Consumption in 3T3-L1 Adipocytes.international journal of molecular sciences,15(3),3481-3494. |
| MLA | Ma, Chengjun,et al."Purification and Characterization of Aporphine Alkaloids from Leaves of Nelumbo nucifera Gaertn and Their Effects on Glucose Consumption in 3T3-L1 Adipocytes".international journal of molecular sciences 15.3(2014):3481-3494. |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。