Characterization of y-type high-molecular-weight glutenins in tetraploid species of Leymus
文献类型：期刊论文
| 作者 | Sun, Yanqi1; Pu, Zongjun2; Dai, Shoufen1; Pu, Xiaoxue1; Liu, Dengcai1,3; Wu, Bihua1; Lan, Xiujin1; Wei, Yuming1; Zheng, Youliang1; Yan, Zehong1 |
| 刊名 | development genes and evolution
 |
| 出版日期 | 2014-02-01 |
| 卷号 | 224期号:1页码:57-64 |
| 关键词 | Gene sequencing Glu-1 Leymus NsXm genome |
| ISSN号 | 0949-944x |
| 中文摘要 | three y-type high-molecular-weight (hmw) glutenin gene open reading frames (orfs), chiy1, chiy2, and racy, were isolated and characterized from leymus chinensis pi499516 and leymus racemosus ssp. racemosus w623305. they shared an extra glutamine in the n-terminal and laaqlpamcrl peptides in the c-terminal with x-type hmw glutenins but had different n-terminal lengths. like other y-type hmw glutenins, chiy2 and racy had 104 (or 105) amino acid (aa) residues at the n-terminal and started with egeasr, whereas chiy1 had 99 aa in this domain and started with qlqcer because of the deletion of egeasr. five other y-type glutenins, including those from elymus ciliaris, pseudoroegneria libanotica, and leymus mollis, were similar to chiy1. the orf of chiy2 was probably not expressed. the orfs of both chiy1 and racy were expressed in bacteria. the maximum likelihood phylogenic tree based on the signal peptide and n-terminal and c-terminal aa residues revealed two clades of y-type hmw glutenins in triticeae; the first contained ay, by, cy, dy, eey, gy, ky, ry, tay, and uy, while the second clade contained the remaining y types, including those from leymus. within the second clade, hmw glutenins lacking the egeasr peptide formed a subclade. these y-type hmw glutenins in leymus could not be targeted to the xm or ns genome. |
| 英文摘要 | three y-type high-molecular-weight (hmw) glutenin gene open reading frames (orfs), chiy1, chiy2, and racy, were isolated and characterized from leymus chinensis pi499516 and leymus racemosus ssp. racemosus w623305. they shared an extra glutamine in the n-terminal and laaqlpamcrl peptides in the c-terminal with x-type hmw glutenins but had different n-terminal lengths. like other y-type hmw glutenins, chiy2 and racy had 104 (or 105) amino acid (aa) residues at the n-terminal and started with egeasr, whereas chiy1 had 99 aa in this domain and started with qlqcer because of the deletion of egeasr. five other y-type glutenins, including those from elymus ciliaris, pseudoroegneria libanotica, and leymus mollis, were similar to chiy1. the orf of chiy2 was probably not expressed. the orfs of both chiy1 and racy were expressed in bacteria. the maximum likelihood phylogenic tree based on the signal peptide and n-terminal and c-terminal aa residues revealed two clades of y-type hmw glutenins in triticeae; the first contained ay, by, cy, dy, eey, gy, ky, ry, tay, and uy, while the second clade contained the remaining y types, including those from leymus. within the second clade, hmw glutenins lacking the egeasr peptide formed a subclade. these y-type hmw glutenins in leymus could not be targeted to the xm or ns genome. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | cell biology ; evolutionary biology ; developmental biology |
| 研究领域[WOS] | cell biology ; evolutionary biology ; developmental biology |
| 关键词[WOS] | subunit genes ; sequences ; genome |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000330719000006 |
| 公开日期 | 2014-12-19 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/4256]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Sichuan Agr Univ, Triticeae Res Inst, Chengdu 611130, Sichuan, Peoples R China 2.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Sichuan, Peoples R China 3.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Yanqi,Pu, Zongjun,Dai, Shoufen,et al. Characterization of y-type high-molecular-weight glutenins in tetraploid species of Leymus[J]. development genes and evolution,2014,224(1):57-64. |
| APA | Sun, Yanqi.,Pu, Zongjun.,Dai, Shoufen.,Pu, Xiaoxue.,Liu, Dengcai.,...&Yan, Zehong.(2014).Characterization of y-type high-molecular-weight glutenins in tetraploid species of Leymus.development genes and evolution,224(1),57-64. |
| MLA | Sun, Yanqi,et al."Characterization of y-type high-molecular-weight glutenins in tetraploid species of Leymus".development genes and evolution 224.1(2014):57-64. |
入库方式： OAI收割
来源：西北高原生物研究所
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