Heterologous gene silencing induced by tobacco rattle virus (TRV) is efficient for pursuing functional genomics studies in woody plants
文献类型：期刊论文
| 作者 | Jiang, Yuanzhong1; Ye, Shenglong1; Wang, Lijun1; Duan, Yanjiao1; Lu, Wanxiang2,3; Liu, Hong1; Fan, Di1; Zhang, Faqi1,3; Luo, Keming1,3 |
| 刊名 | plant cell tissue and organ culture
 |
| 出版日期 | 2014-02-01 |
| 卷号 | 116期号:2页码:163-174 |
| 关键词 | Virus-induced gene silencing Functional genomics Vernicia fordii Populus Nicotiana benthamiana Camellia oleifera |
| ISSN号 | 0167-6857 |
| 中文摘要 | virus-induced gene silencing (vigs) is an effective tool for studying the functions of plant genes, but only a few vigs vectors available for woody plants were reported so far. here we present an effective heterologous vigs system in woody plants based on tobacco rattle virus (trv) vectors. we first tested whether the trv-vector can be directly applied to infect woody plant species, such as vernicia fordii, populus tomentosa carr. and camellia oleifera. the results revealed that trv-mediated vigs could be effectively elicited in v. fordii, weakly in p. tomentosa carr., but not in c. oleifera. trv-based vigs vectors with heterologous phytoene desaturase (pds) sequences from various woody plant species silenced successfully the endogenous pds gene in nicotina benthamiana and v. fordii. the photobleached leaf phenotype of silenced plants significantly correlated with the down-regulation of endogenous pds as compared with controls. to further confirm the reliability of vigs in v. fordii, we also isolated the cloroplastos alterados 1 gene from p. tomentosa carr., and the silencing pheotypes of albino leaves were observed in v. fordii 2 weeks after inoculation using a heterologous trv-based vigs system. taken together, we have successfully developed an agrobacterium-mediated vigs assay in v. fordii and demonstrated that v. fordii as a heterologous vigs system provides a valuable tool for functional genomic analysis in woody plant species. |
| 英文摘要 | virus-induced gene silencing (vigs) is an effective tool for studying the functions of plant genes, but only a few vigs vectors available for woody plants were reported so far. here we present an effective heterologous vigs system in woody plants based on tobacco rattle virus (trv) vectors. we first tested whether the trv-vector can be directly applied to infect woody plant species, such as vernicia fordii, populus tomentosa carr. and camellia oleifera. the results revealed that trv-mediated vigs could be effectively elicited in v. fordii, weakly in p. tomentosa carr., but not in c. oleifera. trv-based vigs vectors with heterologous phytoene desaturase (pds) sequences from various woody plant species silenced successfully the endogenous pds gene in nicotina benthamiana and v. fordii. the photobleached leaf phenotype of silenced plants significantly correlated with the down-regulation of endogenous pds as compared with controls. to further confirm the reliability of vigs in v. fordii, we also isolated the cloroplastos alterados 1 gene from p. tomentosa carr., and the silencing pheotypes of albino leaves were observed in v. fordii 2 weeks after inoculation using a heterologous trv-based vigs system. taken together, we have successfully developed an agrobacterium-mediated vigs assay in v. fordii and demonstrated that v. fordii as a heterologous vigs system provides a valuable tool for functional genomic analysis in woody plant species. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology ; plant sciences |
| 研究领域[WOS] | biotechnology & applied microbiology ; plant sciences |
| 关键词[WOS] | antisense rna ; expression ; arabidopsis ; vectors ; resistance ; sequence ; synthase ; carlavirus ; grapevine ; tomato |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000330386700004 |
| 公开日期 | 2014-12-19 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/4259]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Southwest Univ, Key Lab Ecoenvironm Three Gorges Reservoir Reg, Chongqing Key Lab Transgen Plant & Safety Control, Minist Educ,Inst Resources Bot,Sch Life Sci, Chongqing 400715, Peoples R China 2.Southwest Univ, Coll Hort & Landscape Architecture, Chongqing 400716, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Yuanzhong,Ye, Shenglong,Wang, Lijun,et al. Heterologous gene silencing induced by tobacco rattle virus (TRV) is efficient for pursuing functional genomics studies in woody plants[J]. plant cell tissue and organ culture,2014,116(2):163-174. |
| APA | Jiang, Yuanzhong.,Ye, Shenglong.,Wang, Lijun.,Duan, Yanjiao.,Lu, Wanxiang.,...&Luo, Keming.(2014).Heterologous gene silencing induced by tobacco rattle virus (TRV) is efficient for pursuing functional genomics studies in woody plants.plant cell tissue and organ culture,116(2),163-174. |
| MLA | Jiang, Yuanzhong,et al."Heterologous gene silencing induced by tobacco rattle virus (TRV) is efficient for pursuing functional genomics studies in woody plants".plant cell tissue and organ culture 116.2(2014):163-174. |
入库方式： OAI收割
来源：西北高原生物研究所
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