SEPARATION AND PURIFICATION OF THREE STILBENES FROM THE RADIX OF Polygonum cillinerve (Nakai) Ohwl BY MACROPOROUS RESIN COLUMN CHROMATOGRAPHY COMBINED WITH HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY
文献类型：期刊论文
| 作者 | Chi, Xiaofeng; Xing, Yuxiu; Xiao, Yuancan; Dong, Qi; Hu, Fengzu |
| 刊名 | quimica nova
 |
| 出版日期 | 2014 |
| 卷号 | 37期号:9页码:1465-1468 |
| 关键词 | Polygonum cillinerve (Nakai) Ohwl HSCCC stilbenes |
| ISSN号 | 0100-4042 |
| 中文摘要 | an effective method for the rapid separation and purification of three stilbenes from the radix of polygonum cillinerve (nakai) ohwl by macroporous resin column chromatography combined with high-speed counter-current chromatography (hsccc) was successfully established. in the present study, a two-phase solvent system composed of chloroform-n-butanol-methanol-water (4:1:4:2, v/v/v/v) was used for hsccc separation. a one-step separation in 4 h from 150 mg of crude extract produced 26.3 mg of trans-resveratrol-3-o-glucoside, 42.0 mg of pieceid-2 ''-o-gallate, and 17.9 mg of trans-resveratrol with purities of 99.1%, 97.8%, and 99.4%, respectively, as determined by high-performance liquid chromatography (hplc). the chemical structures of these compounds were identified by nuclear magnetic resonance (nmr) spectroscopy. |
| 英文摘要 | an effective method for the rapid separation and purification of three stilbenes from the radix of polygonum cillinerve (nakai) ohwl by macroporous resin column chromatography combined with high-speed counter-current chromatography (hsccc) was successfully established. in the present study, a two-phase solvent system composed of chloroform-n-butanol-methanol-water (4:1:4:2, v/v/v/v) was used for hsccc separation. a one-step separation in 4 h from 150 mg of crude extract produced 26.3 mg of trans-resveratrol-3-o-glucoside, 42.0 mg of pieceid-2 ''-o-gallate, and 17.9 mg of trans-resveratrol with purities of 99.1%, 97.8%, and 99.4%, respectively, as determined by high-performance liquid chromatography (hplc). the chemical structures of these compounds were identified by nuclear magnetic resonance (nmr) spectroscopy. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | vinifera cell-cultures ; supercritical-fluid extraction ; vitis-vinifera ; preparative separation ; antioxidant activity ; cuspidatum ; lignans ; piceid |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000344472500007 |
| 公开日期 | 2014-12-19 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/4264]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chi, Xiaofeng,Xing, Yuxiu,Xiao, Yuancan,et al. SEPARATION AND PURIFICATION OF THREE STILBENES FROM THE RADIX OF Polygonum cillinerve (Nakai) Ohwl BY MACROPOROUS RESIN COLUMN CHROMATOGRAPHY COMBINED WITH HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY[J]. quimica nova,2014,37(9):1465-1468. |
| APA | Chi, Xiaofeng,Xing, Yuxiu,Xiao, Yuancan,Dong, Qi,&Hu, Fengzu.(2014).SEPARATION AND PURIFICATION OF THREE STILBENES FROM THE RADIX OF Polygonum cillinerve (Nakai) Ohwl BY MACROPOROUS RESIN COLUMN CHROMATOGRAPHY COMBINED WITH HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY.quimica nova,37(9),1465-1468. |
| MLA | Chi, Xiaofeng,et al."SEPARATION AND PURIFICATION OF THREE STILBENES FROM THE RADIX OF Polygonum cillinerve (Nakai) Ohwl BY MACROPOROUS RESIN COLUMN CHROMATOGRAPHY COMBINED WITH HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY".quimica nova 37.9(2014):1465-1468. |
入库方式： OAI收割
来源：西北高原生物研究所
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