Characterization and high-level expression of a metagenome-derived alkaline pectate lyase in recombinant Escherichia coli
文献类型：期刊论文
| 作者 | Wang, Huilin1,2; Li, Xiaoman1,2; Ma, Yanhe1,2,3; Song, Jiangning1,2,4,5 |
| 刊名 | PROCESS BIOCHEMISTRY
 |
| 出版日期 | 2014 |
| 卷号 | 49期号:1页码:69-76 |
| 关键词 | Metagenomic-derived Alkaline pectate lyase Recombinant Escherichia coli Characterization Overproduction |
| 英文摘要 | Alkaline pectate lyases (PLs) play an important role in mild and eco-friendly bioscouring pretreatment processes in the textile industry. However, to date, only a few PLs can be applied in industrial-scale production, and many of them exhibit high production cost, low activity, and/or do not meet the treatment requirements. In this study, an alkaline PL gene was cloned from the metagenomic DNA of alkaline environment soils. The gene pelB consisted of 1263 nucleotides and encoded a mature protein (PelB) of 399 amino acids, which was expressed in Escherichia coli. The maximum catalytic activity of the enzyme exhibited a bimodal distribution at pH 8.1 and 9.8 and an optimal temperature of 55 degrees C. The K-m and V-max values of PelB were 1.78 g/L and 1084.8 mu mol/(L min) at 45 degrees C, respectively. Substrate specificity analysis demonstrated the high cleavage capability of PelB on a broad range of substrates of natural methylated pectin. Based on the degradation products, PelB was considered to be an endo-acting lyase. Using high-cell-density cultivation in 7-L bioreactor, the highest PL activity (1816.2 U/mL) was achieved. Thus, the recombinant PelB, with promising properties for use in bioscouring in the textile pretreatment process, should be a potential enzyme for industrial applications. (C) 2013 Elsevier Ltd, All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | CELL-DENSITY CULTURE ; BACILLUS-SUBTILIS ; POLYGALACTURONATE LYASE ; INDUSTRIAL APPLICATIONS ; SEQUENCE ALIGNMENT ; METHYLATED PECTIN ; BETA-ELIMINATION ; HIGH-THROUGHPUT ; PURIFICATION ; ENZYMES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000331156100010 |
| 公开日期 | 2014-11-23 |
| 源URL | [http://124.16.173.210/handle/311007/430]  |
| 专题 | 天津工业生物技术研究所_结构生物信息学和整合系统生物学实验室 宋江宁_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 3.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China 4.Monash Univ, Fac Med, Dept Biochem & Mol Biol, Melbourne, Vic 3800, Australia 5.Monash Univ, Fac Med, ARC Ctr Excellence Struct & Funct Microbial Gen, Melbourne, Vic 3800, Australia |
| 推荐引用方式 GB/T 7714 | Wang, Huilin,Li, Xiaoman,Ma, Yanhe,et al. Characterization and high-level expression of a metagenome-derived alkaline pectate lyase in recombinant Escherichia coli[J]. PROCESS BIOCHEMISTRY,2014,49(1):69-76. |
| APA | Wang, Huilin,Li, Xiaoman,Ma, Yanhe,&Song, Jiangning.(2014).Characterization and high-level expression of a metagenome-derived alkaline pectate lyase in recombinant Escherichia coli.PROCESS BIOCHEMISTRY,49(1),69-76. |
| MLA | Wang, Huilin,et al."Characterization and high-level expression of a metagenome-derived alkaline pectate lyase in recombinant Escherichia coli".PROCESS BIOCHEMISTRY 49.1(2014):69-76. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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