Distinct structural features of Rex-family repressors to sense redox levels in anaerobes and aerobes
文献类型：期刊论文
| 作者 | Zheng, Yingying1; Ko, Tzu-Ping2; Sun, Hong1; Huang, Chun-Hsiang1; Pei, Jianjun3; Qiu, Riyong3; Wang, Andrew H. -J.2; Wiegel, Juergen4; Shao, Weilan3; Guo, Rey-Ting1 |
| 刊名 | JOURNAL OF STRUCTURAL BIOLOGY
 |
| 出版日期 | 2014-12-01 |
| 卷号 | 188期号:3页码:195-204 |
| 关键词 | Transcription regulation Anaerobic fermentation DNA binding protein Rossmann fold Winged helix domain Allosteric regulation |
| 英文摘要 | The Rex-family repressors sense redox levels by alternative binding to NADH or NAD(+). Unlike other Rex proteins that regulate aerobic respiration, RSP controls ethanol fermentation in the obligate anaerobe Thermoanaerobacter ethanolicus JW200(T). It is also found in other anaerobic microorganisms. Here we present the crystal structures of apo-RSP, RSP/NADH and RSP/NAD(+)/DNA, which are the first structures of Rex-family members from an obligate anaerobe. RSP functions as a homodimer. It assumes an open conformation when bound to the operator DNA and a closed conformation when not DNA-bound. The DNA binds to the N-terminal winged-helix domain and the dinucleotide, either reduced or oxidized, binds to the C-terminal Rossmann-fold domain. The two distinct orientations of nicotinamide ring, anti in NADH and syn in NAD(+), give rise to two sets of protein-ligand interactions. Consequently, NADH binding makes RSP into a closed conformation, which does not bind to DNA. Both the conserved residues and the DNA specificity of RSP show a number of variations from those of the aerobic Rex, reflecting different structural bases for redox-sensing by the anaerobic and aerobic Rex-family members. (C) 2014 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 关键词[WOS] | BACILLUS-SUBTILIS ; ELECTRON-DENSITY ; CRYSTALLOGRAPHY ; PROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000346229500001 |
| 公开日期 | 2014-12-23 |
| 源URL | [http://124.16.173.210/handle/311007/528]  |
| 专题 | 天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China 2.Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan 3.Jiangsu Univ, Sch Environm, Biofuels Inst, Zhenjiang 212013, Peoples R China 4.Univ Georgia, Dept Microbiol, Athens, GA 30602 USA |
| 推荐引用方式 GB/T 7714 | Zheng, Yingying,Ko, Tzu-Ping,Sun, Hong,et al. Distinct structural features of Rex-family repressors to sense redox levels in anaerobes and aerobes[J]. JOURNAL OF STRUCTURAL BIOLOGY,2014,188(3):195-204. |
| APA | Zheng, Yingying.,Ko, Tzu-Ping.,Sun, Hong.,Huang, Chun-Hsiang.,Pei, Jianjun.,...&Guo, Rey-Ting.(2014).Distinct structural features of Rex-family repressors to sense redox levels in anaerobes and aerobes.JOURNAL OF STRUCTURAL BIOLOGY,188(3),195-204. |
| MLA | Zheng, Yingying,et al."Distinct structural features of Rex-family repressors to sense redox levels in anaerobes and aerobes".JOURNAL OF STRUCTURAL BIOLOGY 188.3(2014):195-204. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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