Structural and Functional Analysis of Bacillus subtilis YisP Reveals a Role of Its Product in Biofilm Production
文献类型：期刊论文
| 作者 | Feng, Xinxin1; Hu, Yumei2; Zheng, Yingying2; Zhu, Wei1; Li, Kai1; Huang, Chun-Hsiang2; Ko, Tzu-Ping3; Ren, Feifei2; Chan, Hsiu-Chien2; Nega, Mulugeta4 |
| 刊名 | CHEMISTRY & BIOLOGY
 |
| 出版日期 | 2014-11-20 |
| 卷号 | 21期号:11页码:1557-1563 |
| 英文摘要 | YisP is involved in biofilm formation in Bacillus subtilis and has been predicted to produce C30 isoprenoids. We determined the structure of YisP and observed that it adopts the same fold as squalene and dehydrosqualene synthases. However, the first aspartate-rich motif found in essentially all isoprenoid synthases is aspartate poor in YisP and cannot catalyze head-to-head condensation reactions. We find that YisP acts as a phosphatase, catalyzing formation of farnesol from farnesyl diphosphate, and that it is the first phosphatase to adopt the fold seen in the head-to-head prenyl synthases. Farnesol restores biofilm formation in a Delta yisp mutant and modifies lipid membrane structure similarly to the virulence factor staphyloxanthin. This work clarifies the role of YisP in biofilm formation and suggests an intriguing possibility that many of the YisP-like homologs found in other bacteria may also have interesting products and functions. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | X-RAY-DIFFRACTION ; STAPHYLOCOCCUS-AUREUS ; SQUALENE SYNTHASE ; BIOSYNTHESIS ; MEMBRANES ; FARNESOL ; CHOLESTEROL ; STAPHYLOXANTHIN ; CRYSTALLOGRAPHY ; VIRULENCE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000345506900016 |
| 公开日期 | 2014-12-23 |
| 源URL | [http://124.16.173.210/handle/311007/530]  |
| 专题 | 天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文 |
| 作者单位 | 1.Univ Illinois, Dept Chem, Urbana, IL 61801 USA 2.Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China 3.Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan 4.Univ Tubingen, Interfac Inst Microbiol & Infect Med Tubingen, D-72076 Tubingen, Germany 5.Harvard Univ, Sch Med, Dept Microbiol & Immunol, Boston, MA 02115 USA |
| 推荐引用方式 GB/T 7714 | Feng, Xinxin,Hu, Yumei,Zheng, Yingying,et al. Structural and Functional Analysis of Bacillus subtilis YisP Reveals a Role of Its Product in Biofilm Production[J]. CHEMISTRY & BIOLOGY,2014,21(11):1557-1563. |
| APA | Feng, Xinxin.,Hu, Yumei.,Zheng, Yingying.,Zhu, Wei.,Li, Kai.,...&Oldfield, Eric.(2014).Structural and Functional Analysis of Bacillus subtilis YisP Reveals a Role of Its Product in Biofilm Production.CHEMISTRY & BIOLOGY,21(11),1557-1563. |
| MLA | Feng, Xinxin,et al."Structural and Functional Analysis of Bacillus subtilis YisP Reveals a Role of Its Product in Biofilm Production".CHEMISTRY & BIOLOGY 21.11(2014):1557-1563. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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