Structure, function and inhibition of ent-kaurene synthase from Bradyrhizobium japonicum
文献类型：期刊论文
| 作者 | Liu, Wenting1; Feng, Xinxin2; Zheng, Yingying1; Huang, Chun-Hsiang1; Nakano, Chiaki4; Hoshino, Tsutomu4; Bogue, Shannon2; Ko, Tzu-Ping3; Chen, Chun-Chi1; Cui, Yunfeng1 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2014-10-01 |
| 卷号 | 4期号:6214 |
| 英文摘要 | We report the first X-ray crystal structure of ent-kaur-16-ene synthase from Bradyrhizobium japonicum, together with the results of a site-directed mutagenesis investigation into catalytic activity. The structure is very similar to that of the alpha domains of modern plant terpene cyclases, a result that is of interest since it has been proposed that many plant terpene cyclases may have arisen from bacterial diterpene cyclases. The ent-copalyl diphosphate substrate binds to a hydrophobic pocket near a cluster of Asp and Arg residues that are essential for catalysis, with the carbocations formed on ionization being protected by Leu, Tyr and Phe residues. A bisphosphonate inhibitor binds to the same site. In the kaurene synthase from the moss Physcomitrella patens, 16-alpha-hydroxy-ent-kaurane as well as kaurene are produced since Leu and Tyr in the P. patens kaurene synthase active site are replaced by smaller residues enabling carbocation quenching by water. Overall, the results represent the first structure determination of a bacterial diterpene cyclase, providing insights into catalytic activity, as well as structural comparisons with diverse terpene synthases and cyclases which clearly separate the terpene cyclases from other terpene synthases having highly alpha-helical structures. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | MYCOBACTERIUM-TUBERCULOSIS ; ISOPRENOID BIOSYNTHESIS ; PHYSCOMITRELLA-PATENS ; STRUCTURE PREDICTION ; DITERPENE CYCLASES ; CHEMISTRY ; BIOLOGY ; IDENTIFICATION ; CYCLIZATION ; INSIGHTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000342723400001 |
| 公开日期 | 2014-12-23 |
| 源URL | [http://124.16.173.210/handle/311007/531]  |
| 专题 | 天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China 2.Univ Illinois, Dept Chem, Urbana, IL 61801 USA 3.Acad Sinica, Inst Biol Chem, Taipei 115, Taiwan 4.Niigata Univ, Dept Appl Biol Chem, Niigata 9502181, Japan |
| 推荐引用方式 GB/T 7714 | Liu, Wenting,Feng, Xinxin,Zheng, Yingying,et al. Structure, function and inhibition of ent-kaurene synthase from Bradyrhizobium japonicum[J]. SCIENTIFIC REPORTS,2014,4(6214). |
| APA | Liu, Wenting.,Feng, Xinxin.,Zheng, Yingying.,Huang, Chun-Hsiang.,Nakano, Chiaki.,...&Guo, Rey-Ting.(2014).Structure, function and inhibition of ent-kaurene synthase from Bradyrhizobium japonicum.SCIENTIFIC REPORTS,4(6214). |
| MLA | Liu, Wenting,et al."Structure, function and inhibition of ent-kaurene synthase from Bradyrhizobium japonicum".SCIENTIFIC REPORTS 4.6214(2014). |
入库方式： OAI收割
来源：天津工业生物技术研究所
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