A double-enzyme-coupled assay for high-throughput screening of succinic acid-producing strains
文献类型：期刊论文
| 作者 | Sun, L.1,2; Zhang, H.1; Yuan, H.1; Tu, R.1; Wang, Q.1; Ma, Y.1 |
| 刊名 | JOURNAL OF APPLIED MICROBIOLOGY
 |
| 出版日期 | 2013-06-01 |
| 卷号 | 114期号:6页码:1696-1701 |
| 关键词 | double-enzyme-coupled assay high-throughput screening metabolites microplate assay succinic acid |
| 英文摘要 | Aims To develop a microplate-based high-throughput assay for the detection of succinic acid, which is widely used in many fields of industry. The assay can also be applied to screen high succinic acid-producing strains. Methods and Results Here, we developed a double-enzyme-based succinic acid detection assay using fumarate reductase and peroxidase (named FRDP assay) to screen and isolate high succinic acid-producing bacteria. The assay was performed in 96-well microplate in a high-throughput and had a well linear detection range of succinic acid from 0 center dot 05mmoll1 to 0 center dot 2mmoll1. Interference analysis results showed the complex media (LB),the by-products (formic acid and acetic acid) of fermentation and a longer fermentation time interfere with the FRDP assay, while the mineral media (M9) and short fermentation time (<12h) could minimize the interference. Conclusions The developed double-enzyme-coupled FRDP assay can be used in quick screening of succinic acid production. Significance and Impact of the study In this study, a novel high-throughput screening method to detect succinic acid was developed. This screening method is useful to screen succinic acid-producing bacteria and can be easily adjusted to screen different strains for the production of many other metabolites. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; MANNHEIMIA-SUCCINICIPRODUCENS ; GAS-CHROMATOGRAPHY ; ESCHERICHIA-COLI ; FERMENTATION ; ETHANOL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000319072900014 |
| 公开日期 | 2014-11-23 |
| 源URL | [http://124.16.173.210/handle/311007/410]  |
| 专题 | 天津工业生物技术研究所_进化代谢工程和分子进化研究组 王钦宏_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, L.,Zhang, H.,Yuan, H.,et al. A double-enzyme-coupled assay for high-throughput screening of succinic acid-producing strains[J]. JOURNAL OF APPLIED MICROBIOLOGY,2013,114(6):1696-1701. |
| APA | Sun, L.,Zhang, H.,Yuan, H.,Tu, R.,Wang, Q.,&Ma, Y..(2013).A double-enzyme-coupled assay for high-throughput screening of succinic acid-producing strains.JOURNAL OF APPLIED MICROBIOLOGY,114(6),1696-1701. |
| MLA | Sun, L.,et al."A double-enzyme-coupled assay for high-throughput screening of succinic acid-producing strains".JOURNAL OF APPLIED MICROBIOLOGY 114.6(2013):1696-1701. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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