Molecular identity of axonal sodium channels in human cortical pyramidal cells
文献类型：期刊论文
| 作者 | Tian, CP ; Wang, KY ; Ke, W ; Guo, H ; Shu, YS |
| 刊名 | FRONTIERS IN CELLULAR NEUROSCIENCE
 |
| 出版日期 | 2014 |
| 卷号 | 8期号:1页码:297-297 |
| 关键词 | ACTION-POTENTIAL INITIATION CEREBELLAR PURKINJE NEURONS SEVERE MYOCLONIC EPILEPSY KAINATE-INDUCED SEIZURES CHANDELIER CELLS CEREBRAL-CORTEX VISUAL-CORTEX IN-VIVO MULTIPLE-SCLEROSIS ABSENCE EPILEPSY |
| ISSN号 | 1662-5102 |
| 通讯作者 | Shu, YS (reprint author), Beijing Normal Univ, Sch Brain & Cognit Sci, McGovern Inst Brain Res, State Key Lab Cognit Neurosci & Learning, 19 Xinjiekou Wai St, Beijing 100875, Peoples R China.,yousheng@bnu.edu.cn |
| 英文摘要 | Studies in rodents revealed that selective accumulation of Na+ channel subtypes at the axon initial segment (AIS) determines action potential (AP) initiation and backpropagation in cortical pyramidal cells (PCs); however, in human cortex, the molecular identity of Na+ channels distributed at PC axons, including the AIS and the nodes of Ranvier, remains unclear. We performed immunostaining experiments in human cortical tissues removed surgically to cure brain diseases. We found strong immunosignals of Na+ channels and two channel subtypes, Na-V 1.2 and Na-V 1.6, at the AIS of human cortical PCs. Although both channel subtypes were expressed along the entire AIS, the peak immunosignals of Na-V 1.2 and Na-V 1.6 were found at proximal and distal AIS regions, respectively. Surprisingly, in addition to the presence of Na-V 1.6 at the nodes of Ranvier, Na-V 1.2 was also found in a subpopulation of nodes in the adult human cortex, different from the absence of Na-V 1.2 in myelinated axons in rodents. Na-V 1.1 immunosignals were not detected at either the AIS or the nodes of Ranvier of PCs; however, they were expressed at interneuron axons with different distribution patterns. Further experiments revealed that parvalbumin-positive GABAergic axon cartridges selectively innervated distal AIS regions with relatively high immunosignals of Na-V 1.6 but not the proximal Na-V 1.2-enriched compartments, suggesting an important role of axo-axonic cells in regulating AP initiation in human PCs. Together, our results show that both Na-V 1.2 and Na-V 1.6 (but not Na-V 1.1) channel subtypes are expressed at the AIS and the nodes of Ranvier in adult human cortical PCs, suggesting that these channel subtypes control neuronal excitability and signal conduction in PC axons. |
| 学科主题 | Neurosciences |
| 收录类别 | SCI |
| 资助信息 | 973 Program [2011CBA00400]; National Natural Science Foundation of China [31025012] |
| 语种 | 英语 |
| 公开日期 | 2014-12-15 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/2642]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Tian, CP,Wang, KY,Ke, W,et al. Molecular identity of axonal sodium channels in human cortical pyramidal cells[J]. FRONTIERS IN CELLULAR NEUROSCIENCE,2014,8(1):297-297. |
| APA | Tian, CP,Wang, KY,Ke, W,Guo, H,&Shu, YS.(2014).Molecular identity of axonal sodium channels in human cortical pyramidal cells.FRONTIERS IN CELLULAR NEUROSCIENCE,8(1),297-297. |
| MLA | Tian, CP,et al."Molecular identity of axonal sodium channels in human cortical pyramidal cells".FRONTIERS IN CELLULAR NEUROSCIENCE 8.1(2014):297-297. |
入库方式： OAI收割
来源：上海神经科学研究所
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