Protein tyrosine phosphatase receptor U (PTPRU) is required for glioma growth and motility
文献类型：期刊论文
| 作者 | Zhu, ZC ; Liu, YJ ; Li, K ; Liu, JW ; Wang, HT ; Sun, B ; Xiong, ZQ ; Jiang, HL ; Zheng, J ; Hu, ZL |
| 刊名 | CARCINOGENESIS
 |
| 出版日期 | 2014 |
| 卷号 | 35期号:8页码:1901-1910 |
| 关键词 | FOCAL ADHESION KINASE BETA-CATENIN CELL-MIGRATION CANCER-CELLS IN-VIVO TRANSCRIPTIONAL ACTIVITY BREAST-CANCER COLON-CANCER APC PATHWAY GLIOBLASTOMA |
| ISSN号 | 0143-3334 |
| 通讯作者 | Hu, ZL (reprint author), E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China.,huzelan@ecust.edu.cn |
| 英文摘要 | The membrane protein tyrosine phosphatase receptor U (PTPRU) has been shown to function as a negative regulator of adhesion and proliferation in certain cancer cell types, primarily through its dephosphorylation of beta-catenin and inhibition of subsequent downstream signaling. In the present study, we set out to characterize the role of PTPRU in glioma and found that, while the expression of full-length PTPRU protein is low in these tumors, a number of non-full-length PTPRU isoforms are highly expressed. Among these isoforms, one in particular is localized to the nucleus, and its expression is increased in glioma tissues in a manner that positively correlates with malignancy grade. Short hairpin RNA knockdown of endogenous PTPRU in human and rat glioma cell lines suppressed proliferation, survival, invasion, migration, adhesion and vasculogenic tube formation in vitro, as well as intracranial tumor progression in vivo. In addition, knocking down PTPRU reduced tyrosine phosphorylation (pY) and transcriptional activity of beta-catenin, and we were able to specifically rescue the cell migration defect by expressing a LEF1-beta-catenin fusion protein in PTPRU-depleted cells. PTPRU knockdown also led to increased tyrosine pY of the E3 ubiquitin ligase c-Cbl and to the destabilization of several focal adhesion proteins. Taken together, our findings demonstrate that endogenous PTPRU promote glioma progression through their effect on beta-catenin and focal adhesion signaling. |
| 学科主题 | Oncology |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [31300904]; China Postdoctoral Science Foundation grant [2014M551352]; Exploratory Research Foundation of ECUST; Shanghai Committee of Science and Technology [11DZ2260600] |
| 语种 | 英语 |
| 公开日期 | 2014-12-15 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/2648]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Zhu, ZC,Liu, YJ,Li, K,et al. Protein tyrosine phosphatase receptor U (PTPRU) is required for glioma growth and motility[J]. CARCINOGENESIS,2014,35(8):1901-1910. |
| APA | Zhu, ZC.,Liu, YJ.,Li, K.,Liu, JW.,Wang, HT.,...&Hu, ZL.(2014).Protein tyrosine phosphatase receptor U (PTPRU) is required for glioma growth and motility.CARCINOGENESIS,35(8),1901-1910. |
| MLA | Zhu, ZC,et al."Protein tyrosine phosphatase receptor U (PTPRU) is required for glioma growth and motility".CARCINOGENESIS 35.8(2014):1901-1910. |
入库方式： OAI收割
来源：上海神经科学研究所
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