Clinical Performance of an Automated Stool DNA Assay for Detection of Colorectal Neoplasia
文献类型：期刊论文
| 作者 | Lidgard, GP ; Domanico, MJ ; Bruinsma, JJ ; Light, J ; Gagrat, ZD ; Oldham-Haltom, RL ; Fourrier, KD ; Allawi, H ; Yab, TC ; Taylor, WR ; Simonson, JA ; Devens, M ; Heigh, RI ; Ahlquist, DA ; Berger, BM |
| 刊名 | CLINICAL GASTROENTEROLOGY AND HEPATOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 11期号:10 |
| ISSN号 | 1542-3565 |
| 中文摘要 | BACKGROUND & AIMS: Colorectal cancer (CRC) and advanced precancers can be detected noninvasively by analyses of exfoliated DNA markers and hemoglobin in stool. Practical and cost-effective application of a stool DNA-based (sDNA) test for general CRC screening requires high levels of accuracy and high-capacity throughput. We optimized an automated sDNA assay and evaluated its clinical performance. METHODS: In a blinded, multicenter, case-control study, we collected stools from 459 asymptomatic patients before screening or surveillance colonoscopies and from 544 referred patients. Cases included CRC (n = 93), advanced adenoma (AA) (n = 84), or sessile serrated adenoma 1 cm (SSA) (n = 30); controls included nonadvanced polyps (n = 155) or no colonic lesions (n = 641). Samples were analyzed by using an automated multi-target sDNA assay to measure beta-actin (a marker of total human DNA), mutant KRAS, aberrantly methylated BMP3 and NDRG4, and fecal hemoglobin. Data were analyzed by a logistic algorithmto categorize patients as positive or negative for advanced colorectal neoplasia (CRC, advanced adenoma, and/or SSA 1 cm). RESULTS: At 90% specificity, sDNA analysis identified individuals with CRC with 98% sensitivity. Its sensitivity for stage I cancer was 95%, for stage II cancer it was 100%, for stage III cancer it was 96%, for stage IV cancer it was 100%, and for stages I-III cancers it was 97% (nonsignificant P value). Its sensitivity for advanced precancers (AA and SSA) 1 cm was 57%, for > 2 cm it was 73%, and for > 3 cm it was 83%. The assay detected AA with high-grade dysplasia with 83% sensitivity. CONCLUSIONS: We developed an automated, multi-target sDNA assay that detects CRC and premalignant lesions with levels of accuracy previously demonstrated with a manual process. This automated high-throughput system could be a widely accessible noninvasive approach to general CRC screening. |
| 语种 | 英语 |
| 公开日期 | 2015-01-09 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/5767]  |
| 专题 | 西双版纳热带植物园_文献共享 |
| 推荐引用方式 GB/T 7714 | Lidgard, GP,Domanico, MJ,Bruinsma, JJ,et al. Clinical Performance of an Automated Stool DNA Assay for Detection of Colorectal Neoplasia[J]. CLINICAL GASTROENTEROLOGY AND HEPATOLOGY,2013,11(10). |
| APA | Lidgard, GP.,Domanico, MJ.,Bruinsma, JJ.,Light, J.,Gagrat, ZD.,...&Berger, BM.(2013).Clinical Performance of an Automated Stool DNA Assay for Detection of Colorectal Neoplasia.CLINICAL GASTROENTEROLOGY AND HEPATOLOGY,11(10). |
| MLA | Lidgard, GP,et al."Clinical Performance of an Automated Stool DNA Assay for Detection of Colorectal Neoplasia".CLINICAL GASTROENTEROLOGY AND HEPATOLOGY 11.10(2013). |
入库方式： OAI收割
来源：西双版纳热带植物园
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