Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry
文献类型：期刊论文
| 作者 | Kang TF(康腾飞) ; Niu Y(牛宇); Jin G(靳刚)
|
| 刊名 | Thin Solid Films
 |
| 出版日期 | 2014-11-28 |
| 卷号 | 571页码:463-467 |
| 通讯作者邮箱 | gajin@imech.ac.cn |
| 关键词 | Biosensor Total internal reflection imaging ellipsometry Weak affinity interaction Tris Lysozyme |
| ISSN号 | 0040-6090 |
| 产权排序 | [Kang, Tengfei; Niu, Yu; Jin, Gang] Chinese Acad Sci, Inst Mech, NML, Beijing 100190, Peoples R China; [Kang, Tengfei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 通讯作者 | Jin, G (reprint author), 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China. |
| 合作状况 | 国内 |
| 中文摘要 | Weak affinity interactions among biomolecules have attracted more and more attentions for they play a significant role in organisms. However, it is difficult to characterize these interactions due to the limitation of the detection approaches. The biosensor based on total internal reflection imaging ellipsometry (TIRIE) is a real-time analysis method for biomolecular interaction detection with the advantages of high throughput and sensitivity. The interaction between tris and lysozyme (LZM), a typical example of weak affinity interactions, has been detected with TIRIE as a trial. Tris is immobilized on the gold thin film substrate to form the biosensing surface and then LZM as well as its negative controls is delivered to the tris-immobilized surface. The interaction process between tris and LZM is recorded by TIRIE biosensor to forma real-time curve and its dissociation constant is deduced as 7.6 x 10(-5) M, which is in agreement with the results in the reported work by other investigators. The results indicate that TIRIE biosensor is competent for weak affinity interaction analysis. (C) 2014 Elsevier B.V. All rights reserved. |
| 学科主题 | Materials Science ; Physics |
| 分类号 | 二类/Q2 |
| 类目[WOS] | Materials Science, Multidisciplinary ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter |
| 研究领域[WOS] | Materials Science ; Physics |
| 关键词[WOS] | PLASMON RESONANCE BIOSENSOR ; POLARIZATION ; RECOGNITION ; AFFINITIES ; ANTIGEN |
| 收录类别 | SCI ; EI |
| 原文出处 | http://dx.doi.org/10.1016/j.tsf.2014.02.098 |
| 语种 | 英语 |
| WOS记录号 | WOS:000346055200022 |
| 公开日期 | 2015-01-23 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/49493]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Kang TF,Niu Y,Jin G. Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry[J]. Thin Solid Films,2014,571:463-467. |
| APA | Kang TF,Niu Y,&Jin G.(2014).Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry.Thin Solid Films,571,463-467. |
| MLA | Kang TF,et al."Visualization of the interaction between tris and lysozyme with a biosensor based on total internal reflection imaging ellipsometry".Thin Solid Films 571(2014):463-467. |
入库方式： OAI收割
来源：力学研究所
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