Real-time PCR targeting OmpA gene for detection of Cronobacter spp. in powdered infant formula
文献类型：期刊论文
| 作者 | [Dong, Xiaohui ; Wu, Qingping ; Wu, Kui] Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China ; [Dong, Xiaohui ; Wu, Qingping ; Wu, Kui ; Zhang, Jumei] Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbial Culture Collect, Guangdong Open Lab Appl Microbiol, State Key Lab Appl Microbiol, Guangzhou 510070, Guangdong, Peoples R China ; [Dong, Xiaohui ; Wu, Kui] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 刊名 | FOOD SCIENCE AND BIOTECHNOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 22期号:2页码:309-313 |
| 关键词 | Cronobacter spp. real-time PCR TaqMan ompA detection |
| ISSN号 | 1226-7708 |
| 通讯作者 | wuqp203@yahoo.com.cn |
| 中文摘要 | Cronobacter spp. (formerly Enterobacter sakazakii), a pathogen commonly found in powdered infant formula (PIF), is a rare cause of invasive infection with a high mortality rate in neonates. In the present study, a realtime PCR assay was conducted to identify the pathogens in PIF using a TaqMan probe targeting the outer membrane protein A gene (ompA) of Cronobacter spp. The specificity of the PCR assay was tested against 25 strains of Cronobacter spp. and 38 non-Cronobacter bacterial species. The detection limits of this method are 1.0x10(2) copy/mu L in standard plasmid, 1.1 CFU/100 g in PIF through 38 h of enrichment, and 2.8x10(2) CFU/mL in phosphate-buffered saline (pH 7.0). Based on the detection limits, real-time PCR is more sensitive than simplex and duplex PCR. These methods were successfully applied to actual samples, indicating that this real-time PCR assay can be used for the detection of Cronobacter spp. in PIF. |
| 学科主题 | Food Science & Technology |
| 资助信息 | We acknowledge the financing support of the Guangdong-Hongkong Major Breakthrough Project in Key Fields (2010498D10008) and Science and Technology Planning Project of Guangzhou (2010u1-E00611). |
| 原文出处 | KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST |
| 语种 | 英语 |
| WOS记录号 | WOS:000318411900003 |
| 公开日期 | 2015-01-14 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/10920]  |
| 专题 | 南海海洋研究所_热带海洋环境国家重点实验室(LTO) |
| 推荐引用方式 GB/T 7714 | [Dong, Xiaohui,Wu, Qingping,Wu, Kui] Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China,et al. Real-time PCR targeting OmpA gene for detection of Cronobacter spp. in powdered infant formula[J]. FOOD SCIENCE AND BIOTECHNOLOGY,2013,22(2):309-313. |
| APA | [Dong, Xiaohui.,Wu, Qingping.,Wu, Kui] Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China.,[Dong, Xiaohui.,Wu, Qingping.,...&Wu, Kui] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.(2013).Real-time PCR targeting OmpA gene for detection of Cronobacter spp. in powdered infant formula.FOOD SCIENCE AND BIOTECHNOLOGY,22(2),309-313. |
| MLA | [Dong, Xiaohui,et al."Real-time PCR targeting OmpA gene for detection of Cronobacter spp. in powdered infant formula".FOOD SCIENCE AND BIOTECHNOLOGY 22.2(2013):309-313. |
入库方式： OAI收割
来源：南海海洋研究所
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