Insights into Caerulomycin A Biosynthesis: A Two-Component Monooxygenase CrmH-Catalyzed Oxime Formation
文献类型：期刊论文
| 作者 | [Zhu, Yiguang ; Zhang, Qingbo ; Li, Sumei ; Lin, Qinheng ; Zhang, Guangtao ; Zhang, Haibo ; Zhang, Changsheng] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, RNAM Ctr Marine Microbiol, Guangdong Key Lab Marine Mat Med,South China Sea, Guangzhou 510301, Guangdong, Peoples R China ; [Zhang, Qingbo ; Lin, Qinheng] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Fu, Peng ; Zhu, Weiming] Ocean Univ China, Sch Med & Pharm, Key Lab Marine Drugs, Chinese Minist Educ, Qingdao 266003, Peoples R China ; [Shi, Rong] Univ Laval, Dept Biochim Microbiol & Bioinformat, PROTEO, Quebec City, PQ G1V 0A6, Canada ; [Shi, Rong] Univ Laval, IBIS, Quebec City, PQ G1V 0A6, Canada |
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2013 |
| 卷号 | 135期号:50页码:18750-18753 |
| ISSN号 | 0002-7863 |
| 其他题名 | mannual of IR1 |
| 产权排序 | 产权排序 |
| 通讯作者 | weimingzhu@ouc.edu.cn ; czhang2006@gmail.com |
| 合作状况 | 填入合作性质等 |
| 中文摘要 | The immunosuppressive agent caerulomycin A features a unique 2,2'-bipyridine core structure and an unusual oxime functionality. Genetic and biochemical evidence confirms that the oxime formation in caerulomycin A biosynthesis is catalyzed by CrmH, a flavin-dependent two-component monooxygenase that is compatible with multiple flavin reductases, from a primary amine via a N-hydroxylamine intermediate. Structure homologue-guided site-directed mutagenesis studies identify four amino acid residues that are essential for CrmH catalysis. This study provides the first biochemical evidence of a two-component monooxygenase that catalyzes oxime formation. |
| 英文摘要 | description.abstract |
| 学科主题 | Chemistry, Multidisciplinary |
| 收录类别 | SCI |
| 资助信息 | Financial support was provided by MOST (2010CB833805), NSFC (31125001 and 31070045), and CAS (KZCX2-YW-JC202, KSCX2-EW-G-12, 08SL111002). We thank Profs. Yi Tang (UCLA), Shu-Ming Li (Philipps Universitat Marburg), and Shuangjun Lin (Shanghai Jiao Tong University) for help discussions. We are grateful to the analytical facilities in SCSIO. |
| 原文出处 | AMER CHEMICAL SOC |
| 语种 | 英语 |
| WOS记录号 | WOS:000328865100012 |
| 公开日期 | 2015-01-22 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/10944]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | [Zhu, Yiguang,Zhang, Qingbo,Li, Sumei,et al. Insights into Caerulomycin A Biosynthesis: A Two-Component Monooxygenase CrmH-Catalyzed Oxime Formation[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2013,135(50):18750-18753. |
| APA | [Zhu, Yiguang.,Zhang, Qingbo.,Li, Sumei.,Lin, Qinheng.,Zhang, Guangtao.,...&[Shi, Rong] Univ Laval, IBIS, Quebec City, PQ G1V 0A6, Canada.(2013).Insights into Caerulomycin A Biosynthesis: A Two-Component Monooxygenase CrmH-Catalyzed Oxime Formation.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,135(50),18750-18753. |
| MLA | [Zhu, Yiguang,et al."Insights into Caerulomycin A Biosynthesis: A Two-Component Monooxygenase CrmH-Catalyzed Oxime Formation".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 135.50(2013):18750-18753. |
入库方式： OAI收割
来源：南海海洋研究所
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