Cloning, characterization and expression analysis of a caspase-8 like gene from the Hong Kong oyster, Crassostrea hongkongensis
文献类型：期刊论文
| 作者 | [Xiang, Zhiming ; Qu, Fufa ; Zhang, Yang ; Tong, Ying ; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Qu, Fufa] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Qi, Lin] Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 35期号:6页码:1797-1803 |
| 关键词 | Crassostrea hongkongensis Apoptosis ChCaspase-8 NF-kappa B |
| ISSN号 | 1050-4648 |
| 通讯作者 | carlzyu@scsio.ac.cn |
| 中文摘要 | Apoptosis plays a key role in many biological processes, including homeostasis within the immune system. A family of cysteine proteases, the caspases, constitutes the core of the apoptotic machinery. We have characterized the first bivalve caspase-8 ortholog from the Hong Kong oyster Crassostrea hongkongensis (designated ChCaspase-8). The full-length cDNA is 1945 bp in length encoding a putative protein of 557 amino acids that contains two N-terminal DED domains, and a CASc domain at the C-terminus. ChCaspase-8 is ubiquitously expressed in oysters, with highest expression levels in the gonad and labial palps. Following microbial infection, the expression of ChCaspase-8 increased in hemocytes from 12 to 72 h post-challenge. When expressed in HeLa cells, ChCaspase-8 is located in the cytoplasm, while over-expression of ChCaspase-8 in HEK293T cells activates the transcriptional activities of NF-kappa B. These results indicate that ChCaspase-8 might play an important role in the immune and apoptotic responses of oysters. (C) 2013 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 资助信息 | This research was supported by the National Science Foundation of China (No. 31202021 and 41176150), a National Basic Research Program of China (No. 2010CB126404) and a Guangdong-NSFC Joint Fund (U1201215). We thank Prof. Elizabeth De Stasio for her editing to the manuscript. |
| 原文出处 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 语种 | 英语 |
| WOS记录号 | WOS:000330158800012 |
| 公开日期 | 2015-01-22 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/10950]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | [Xiang, Zhiming,Qu, Fufa,Zhang, Yang,et al. Cloning, characterization and expression analysis of a caspase-8 like gene from the Hong Kong oyster, Crassostrea hongkongensis[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(6):1797-1803. |
| APA | [Xiang, Zhiming.,Qu, Fufa.,Zhang, Yang.,Tong, Ying.,Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.,...&[Qi, Lin] Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China.(2013).Cloning, characterization and expression analysis of a caspase-8 like gene from the Hong Kong oyster, Crassostrea hongkongensis.FISH & SHELLFISH IMMUNOLOGY,35(6),1797-1803. |
| MLA | [Xiang, Zhiming,et al."Cloning, characterization and expression analysis of a caspase-8 like gene from the Hong Kong oyster, Crassostrea hongkongensis".FISH & SHELLFISH IMMUNOLOGY 35.6(2013):1797-1803. |
入库方式： OAI收割
来源：南海海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。