Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication
文献类型：期刊论文
| 作者 | [Fu, Jian Ping ; Luo, Zhang ; Zou, Peng Fei ; Zhang, Shu Huan ; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China ; [Fu, Jian Ping] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Yan, Yang ; Qin, Qi Wei] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Nie, Pin] Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China |
| 刊名 | FISH PATHOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 48期号:4页码:126-134 |
| ISSN号 | 0388-788X |
| 关键词 | cell line Pelodiscus sinensis Chinese soft-shelled turtle transfection soft-shelled turtle iridovirus STIV |
| 通讯作者 | pinnie@ihb.ac.cn |
| 中文摘要 | continuous cell line derived from extracranial carotid artery of the Chinese soft-shelled turtle Pelodiscus sinensis was established and characterized. It was named as STA (soft-shelled turtle artery) cell line. The cells were muscle-cell like, and were stained with antibody against smooth muscle myofilament marker, a-smooth muscle actin. These cells had a normal diploid chromosome number of 2n = 66, and their 12S rRNA and 16S rRNA sequences shared 99% identity with ones of the turtle in database. The cell line can be cultured well in media DMEM/F12 or M199 supplemented with 10% FBS at 28 C. It was further demonstrated that the cells were transfected successfully with pTurbo plasmid, with a transfection efficiency being over 30%. The soft-shelled turtle iridovirus (STIV) propagated in the cell line, causing typical CPE with the formation of inclusion bodies. It is suggested that the established STA cell line provides a convenient platform for studying the pathogenesis of STIV and biological aspects of the turtle. |
| 学科主题 | Fisheries ; Veterinary Sciences |
| 资助信息 | The research was financially supported by a grant (31230075) from the National Natural Science Foundation of China and a grant (2009CB118703) from National Basic Research of Program (973 Program). |
| 原文出处 | JAPAN SOC FISH PATHOL DEPT FISHERIES-FAC AGR |
| 语种 | 英语 |
| WOS记录号 | WOS:000329960200003 |
| 公开日期 | 2015-01-22 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/10953]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | [Fu, Jian Ping,Luo, Zhang,Zou, Peng Fei,et al. Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication[J]. FISH PATHOLOGY,2013,48(4):126-134. |
| APA | [Fu, Jian Ping.,Luo, Zhang.,Zou, Peng Fei.,Zhang, Shu Huan.,Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.,...&[Nie, Pin] Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China.(2013).Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication.FISH PATHOLOGY,48(4),126-134. |
| MLA | [Fu, Jian Ping,et al."Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication".FISH PATHOLOGY 48.4(2013):126-134. |
入库方式： OAI收割
来源：南海海洋研究所
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