Molecular identification of insulin-related peptide receptor and its potential role in regulating development in Pinctada fucata
文献类型：期刊论文
| 作者 | [Shi, Yu ; Guan, Yunyan ; He, Maoxian] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2013 |
| 卷号 | 408页码:118-127 |
| 关键词 | Insulin-related peptide receptor PQ401 Insulin-like growth factor-I Development Pinctada fucata |
| ISSN号 | 0044-8486 |
| 通讯作者 | hmx@scsio.ac.cn |
| 中文摘要 | The insulin-like family is not only an important regulatory factor for animal growth, development, and metabolism, but also a mediator for initiating growth activity of growth hormone. It plays an important role in transferring transmembrane information and regulating cell function by binding to tyrosine kinase receptors (insulin receptors). To better understand the role of insulin-related peptide receptor (Pfirr) on the developmental regulation in Pinctada fucata, 5.326 kb encoding cDNAs for Pfirr have been cloned and functionally characterized. Pfirr displays significant homologies to Crassostrea gigas, and exhibits all the typical features of insulin receptors and tyrosine kinase domain structure, both of which are typical for the protein family sharing high similarity to other orthologs. Real-time PCR analyses show that Pfirr widely expresses in tissues and developmental stages of P. fucata. Expression of Pfirr mRNAs at different developmental stages (polar body stage, the trocophore stage and D-shaped larva stage) following treatment with agonist IGF-I(1, 2, 4 and 8 mu M/L) and antagonist PQ401 (5, 15, 25, 50 and 100 mu M/L) indicated that Pfirr may be involved in regulating the development of embryos in P. fucata. These results clearly demonstrate Pfirr is involved in regulating developmental process in P. fucata. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved. |
| 学科主题 | Fisheries ; Marine & Freshwater Biology |
| 资助信息 | We thank Mr. Manhong Wang for valuable assistance in rearing the oysters and Qi Wang for assistance in collecting samples. This project was supported by the National Natural Science Foundation of China (41176145), the Joint Program of NSFC-Guangdong (U0831001), and the Funds of Knowledge Innovation Program of the Chinese Academy of Sciences (ZCX2-EW-Q21). |
| 原文出处 | ELSEVIER SCIENCE BV |
| 语种 | 英语 |
| WOS记录号 | WOS:000323554900016 |
| 公开日期 | 2015-01-22 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/10985]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | [Shi, Yu,Guan, Yunyan,He, Maoxian] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China. Molecular identification of insulin-related peptide receptor and its potential role in regulating development in Pinctada fucata[J]. AQUACULTURE,2013,408:118-127. |
| APA | [Shi, Yu,Guan, Yunyan,&He, Maoxian] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China.(2013).Molecular identification of insulin-related peptide receptor and its potential role in regulating development in Pinctada fucata.AQUACULTURE,408,118-127. |
| MLA | [Shi, Yu,et al."Molecular identification of insulin-related peptide receptor and its potential role in regulating development in Pinctada fucata".AQUACULTURE 408(2013):118-127. |
入库方式： OAI收割
来源：南海海洋研究所
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