High mobility group box 1 can enhance NF-kappa B activation and act as a pro-inflammatory molecule in the Pacific oyster, Crassostrea gigas
文献类型：期刊论文
| 作者 | [Li, Jun ; Zhang, Yang ; Xiang, Zhiming ; Xiao, Shu ; Yu, Feng ; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Lab Appl Marine Biol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China ; [Li, Jun] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 35期号:1页码:63-70 |
| 关键词 | Crassostrea gigas HMGB1 NF-kappa B Sub-cellular localization Pro-inflammatory cytokine |
| ISSN号 | 1050-4648 |
| 通讯作者 | carlzyu@scsio.ac.cnv |
| 中文摘要 | High-mobility group box 1 (HMGB1), a highly conserved DNA-binding protein, is involved in nucleosome formation and transcriptional regulation, and can also act as an extracellular cytokine to trigger inflammation and immune responses. In this study, we identified a HMGB1 gene (hereafter designated as CgHMGB1) in the Pacific oyster Crassostrea gigas. The full-length CgHMGB1 cDNA is 833 bp including 5' and 3'-untranslated regions (UTRs) of 145 and 79 bp, respectively, and an open reading frame (ORF) of 609 bp. The gene encodes a 202 amino acid polypeptide with an estimated molecular mass of 23.3 kDa. Sequence alignment shows that CgHMGB1 contains two basic HMG boxes and a highly acidic C-terminal domain. Recombinant CgHMGB1 proteins can enhance the mRNA level of various inflammatory cytokines in vivo. Typically, CgHMGB1 is localized in the nucleus, though lipopolysaccharide can induce its release to cytoplasm. Moreover, luciferase reporter assays reveal that CgHMGB1 cannot stimulate Nuclear Factor-kappa B reporter activity alone, but it can enhance Rel-dependent NF-kappa B activation in a dose-dependent manner. CgHMGB1 is highly expressed in hemocytes and its transcripts are significantly more abundant following bacterial challenge. Our results suggest that CgHMGB1 plays an essential role in innate defense by enhancing Rel-activated NF-kappa B activity and inducing the expression of inflammatory cytokines. (c) 2013 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 资助信息 | This work was supported by a National Basic Research Program of China (No. 2010CB126404) and National Science Foundation of China (No. 31001130 and 41176150). We also thank Prof. Elizabeth De Stasio for critical editing of the manuscript. |
| 原文出处 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 语种 | 英语 |
| WOS记录号 | WOS:000320749700008 |
| 公开日期 | 2015-01-22 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/11015]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | [Li, Jun,Zhang, Yang,Xiang, Zhiming,et al. High mobility group box 1 can enhance NF-kappa B activation and act as a pro-inflammatory molecule in the Pacific oyster, Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(1):63-70. |
| APA | [Li, Jun.,Zhang, Yang.,Xiang, Zhiming.,Xiao, Shu.,Yu, Feng.,...&[Li, Jun] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China.(2013).High mobility group box 1 can enhance NF-kappa B activation and act as a pro-inflammatory molecule in the Pacific oyster, Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,35(1),63-70. |
| MLA | [Li, Jun,et al."High mobility group box 1 can enhance NF-kappa B activation and act as a pro-inflammatory molecule in the Pacific oyster, Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 35.1(2013):63-70. |
入库方式： OAI收割
来源：南海海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。