Allograft inflammatory factor-1 stimulates hemocyte immune activation by enhancing phagocytosis and expression of inflammatory cytokines in Crassostrea gigas
文献类型：期刊论文
| 作者 | [Zhang, Yang ; Li, Jun ; Yu, Feng ; He, Xiaocui ; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Lab Appl Marine Biol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 34期号:5页码:1071-1077 |
| 关键词 | Crassostrea gigas AIF-1 Hemocyte activation Phagocytosis Inflammatory cytokine |
| ISSN号 | 1050-4648 |
| 通讯作者 | carlzyu@scsio.ac.cn |
| 中文摘要 | Allograft inflammatory factor-1 (AIF-1) is a calcium-binding cytokine associated with immune cell activation and inflammatory response. Presently, we have identified and characterized an AIF-1 in a marine bivalve mollusk, Crassostrea gigas, and designated it as CgAIF-1. The full-length CgAIF-1 cDNA is 794 bp, encoding a protein of 149 amino acids with two conserved EF hand Ca2+-binding motifs. CgAIF-1 is constitutively expressed in various tissues with enriched expression in hemocytes. Moreover, CgAIF-1 transcription is induced by multiple Pathogen-Associated Molecular Patterns (PAMPs), including poly (I: C), LPS, PGN, HKLM and HKVA, but is limited by 1,3-beta-glucan. Furthermore, recombinant CgAIF-1 can specifically stimulate phagocytic ability of granulocytes, but not of intermediate cells and hyalinocytes. CgAIF-1 also enhances mRNA levels of MIF, TNF and IL-17. These results provide the first functional evidence that CgAIF-1 is involved in hemocyte activation in C gigas, revealing conserved functions of AIF-1 in host defense from mollusks to mammals. (C) 2013 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 资助信息 | This work was supported by the National Science Foundation of China (No. 31001130 and 41176150), the National Basic Research Program of China (No. 2010CB126404) and the Joint Funds of NSFC-Guangdong of China (U1201215). We also thank Prof. Elizabeth De Stasio for critical reading and editing of the manuscript. |
| 原文出处 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 语种 | 英语 |
| WOS记录号 | WOS:000317870600007 |
| 公开日期 | 2015-01-22 |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/11038]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | [Zhang, Yang,Li, Jun,Yu, Feng,et al. Allograft inflammatory factor-1 stimulates hemocyte immune activation by enhancing phagocytosis and expression of inflammatory cytokines in Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(5):1071-1077. |
| APA | [Zhang, Yang,Li, Jun,Yu, Feng,He, Xiaocui,&Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Lab Appl Marine Biol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China.(2013).Allograft inflammatory factor-1 stimulates hemocyte immune activation by enhancing phagocytosis and expression of inflammatory cytokines in Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,34(5),1071-1077. |
| MLA | [Zhang, Yang,et al."Allograft inflammatory factor-1 stimulates hemocyte immune activation by enhancing phagocytosis and expression of inflammatory cytokines in Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 34.5(2013):1071-1077. |
入库方式： OAI收割
来源：南海海洋研究所
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