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Acetylome Analysis Reveals Diverse Functions of Lysine Acetylation in Mycobacterium tuberculosis
文献类型：期刊论文
| 作者 | Liu, Fengying1; Yang, Mingkun2; Wang, Xude1; Yang, Shanshan1; Gu, Jing1; Zhou, Jie3; Zhang, Xian-En4; Deng, Jiaoyu1; Ge, Feng2 |
| 刊名 | MOLECULAR & CELLULAR PROTEOMICS
 |
| 出版日期 | 2014-12-01 |
| 卷号 | 13期号:12页码:3352-3366 |
| ISSN号 | 1535-9476 |
| 关键词 | TANDEM MASS-SPECTROMETRY BACTERIUM BACILLUS-SUBTILIS M-BOVIS BCG ESCHERICHIA-COLI COA SYNTHETASE SACCHAROMYCES-CEREVISIAE PHOSPHOPROTEOME ANALYSIS PROTEIN IDENTIFICATION REVERSIBLE ACETYLATION PLASMODIUM-FALCIPARUM |
| 英文摘要 | The lysine acetylation of proteins is a reversible post-translational modification that plays a critical regulatory role in both eukaryotes and prokaryotes. Mycobacterium tuberculosis is a facultative intracellular pathogen and the causative agent of tuberculosis. Increasing evidence shows that lysine acetylation may play an important role in the pathogenesis of M. tuberculosis. However, only a few acetylated proteins of M. tuberculosis are known, presenting a major obstacle to understanding the functional roles of reversible lysine acetylation in this pathogen. We performed a global acetylome analysis of M. tuberculosis H37Ra by combining protein/peptide prefractionation, antibody enrichment, and LC-MS/MS. In total, we identified 226 acetylation sites in 137 proteins of M. tuberculosis H37Ra. The identified acetylated proteins were functionally categorized into an interaction map and shown to be involved in various biological processes. Consistent with previous reports, a large proportion of the acetylation sites were present on proteins involved in glycolysis/gluconeogenesis, the citrate cycle, and fatty acid metabolism. A NAD(+)-dependent deacetylase (MRA_1161) deletion mutant of M. tuberculosis H37Ra was constructed and its characterization showed a different colony morphology, reduced biofilm formation, and increased tolerance of heat stress. Interestingly, lysine acetylation was found, for the first time, to block the immunogenicity of a peptide derived from a known immunogen, HspX, suggesting that lysine acetylation plays a regulatory role in immunogenicity. Our data provide the first global survey of lysine acetylation in M. tuberculosis. The dataset should be an important resource for the functional analysis of lysine acetylation in M. tuberculosis and facilitate the clarification of the entire metabolic networks of this life-threatening pathogen. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | TANDEM MASS-SPECTROMETRY ; BACTERIUM BACILLUS-SUBTILIS ; M-BOVIS BCG ; ESCHERICHIA-COLI ; COA SYNTHETASE ; SACCHAROMYCES-CEREVISIAE ; PHOSPHOPROTEOME ANALYSIS ; PROTEIN IDENTIFICATION ; REVERSIBLE ACETYLATION ; PLASMODIUM-FALCIPARUM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000345626400011 |
| 公开日期 | 2015-01-20 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/20372]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China 3.Foshan Fourth Peoples Hosp, Foshan, Peoples R China 4.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Fengying,Yang, Mingkun,Wang, Xude,et al. Acetylome Analysis Reveals Diverse Functions of Lysine Acetylation in Mycobacterium tuberculosis[J]. MOLECULAR & CELLULAR PROTEOMICS,2014,13(12):3352-3366. |
| APA | Liu, Fengying.,Yang, Mingkun.,Wang, Xude.,Yang, Shanshan.,Gu, Jing.,...&Ge, Feng.(2014).Acetylome Analysis Reveals Diverse Functions of Lysine Acetylation in Mycobacterium tuberculosis.MOLECULAR & CELLULAR PROTEOMICS,13(12),3352-3366. |
| MLA | Liu, Fengying,et al."Acetylome Analysis Reveals Diverse Functions of Lysine Acetylation in Mycobacterium tuberculosis".MOLECULAR & CELLULAR PROTEOMICS 13.12(2014):3352-3366. |
入库方式： OAI收割
来源：水生生物研究所
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