Expression pattern of somatolactin in the Chinese sturgeon, Acipenser sinensis Gray, 1835
文献类型：期刊论文
| 作者 | Leng, X. -Q.1; Li, C. -J.2; Cao, H.1 |
| 刊名 | JOURNAL OF APPLIED ICHTHYOLOGY
 |
| 出版日期 | 2014-12-01 |
| 卷号 | 30期号:6页码:1222-1228 |
| 关键词 | MESSENGER-RNA EXPRESSION BREAM SPARUS-AURATA GROWTH-HORMONE PITUITARY PROTEIN CDNA CLONING MOLECULAR CHARACTERIZATION PHYLOGENETIC ANALYSIS FISH GONADOTROPINS RAINBOW-TROUT PLASMA-LEVELS |
| ISSN号 | 0175-8659 |
| 英文摘要 | Somatolactin (SL) is a pituitary hormone specific to fish and is reported in a variety of teleost species. Although it appears to be involved in many different physiological processes, its biological functions are poorly understood in Acipenseriformes. In this paper the expression pattern of this protein is characterized in Chinese sturgeon (Acipenser sinensis). Structure analysis showed that AsSL had a 24aa signal peptide and a 208aa mature protein, one N-glycosylation site at positions 145-147 and seven Cys residues conserved with other SLs. The antiserum was raised against a 15 aa antigen, derived from the Chinese sturgeon somatolactin (AsSL) amino acid sequence. Western blot analysis showed the pituitary-specific expression of AsSL. In situ hybridization revealed that the mRNA of AsSL was expressed mainly in pars intermedia (PI), although also slightly in proximal pars distalis (PPD), cells expressing AsSL disperse throughout the pars intermedia. These findings provide useful information for future physiological research in Chinese sturgeon. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Marine & Freshwater Biology |
| 研究领域[WOS] | Fisheries ; Marine & Freshwater Biology |
| 关键词[WOS] | MESSENGER-RNA EXPRESSION ; BREAM SPARUS-AURATA ; GROWTH-HORMONE ; PITUITARY PROTEIN ; CDNA CLONING ; MOLECULAR CHARACTERIZATION ; PHYLOGENETIC ANALYSIS ; FISH GONADOTROPINS ; RAINBOW-TROUT ; PLASMA-LEVELS |
| 收录类别 | SCI ; ISTP |
| 语种 | 英语 |
| WOS记录号 | WOS:000345588500018 |
| 公开日期 | 2015-01-20 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/20375]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grad Sch, Wuhan 430072, Peoples R China 2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat & Utilizat, Agr Minist China, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Leng, X. -Q.,Li, C. -J.,Cao, H.. Expression pattern of somatolactin in the Chinese sturgeon, Acipenser sinensis Gray, 1835[J]. JOURNAL OF APPLIED ICHTHYOLOGY,2014,30(6):1222-1228. |
| APA | Leng, X. -Q.,Li, C. -J.,&Cao, H..(2014).Expression pattern of somatolactin in the Chinese sturgeon, Acipenser sinensis Gray, 1835.JOURNAL OF APPLIED ICHTHYOLOGY,30(6),1222-1228. |
| MLA | Leng, X. -Q.,et al."Expression pattern of somatolactin in the Chinese sturgeon, Acipenser sinensis Gray, 1835".JOURNAL OF APPLIED ICHTHYOLOGY 30.6(2014):1222-1228. |
入库方式： OAI收割
来源：水生生物研究所
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