Functional Divergence of the miRNA Transcriptome at the Onset of Drosophila Metamorphosis
文献类型：期刊论文
| 作者 | Yeh, Shu-Dan1; von Grotthuss, Marcin1; Gandasetiawan, Kania A.1; Jayasekera, Suvini1; Xia, Xiao-Qin2; Chan, Carolus1; Jayaswal, Vivek3; Ranz, Jose M.1 |
| 刊名 | MOLECULAR BIOLOGY AND EVOLUTION
 |
| 出版日期 | 2014-10-01 |
| 卷号 | 31期号:10页码:2557-2572 |
| 关键词 | Drosophila evolution of expression profiles miRNAs metamorphosis miRNA-mRNA associations |
| ISSN号 | 0737-4038 |
| 英文摘要 | MicroRNAs (miRNAs) are endogenous RNA molecules that regulate gene expression posttranscriptionally. To date, the emergence ofmiRNAs and their patterns of sequence evolution have been analyzed in great detail. However, the extent to which miRNA expression levels have evolved over time, the role different evolutionary forces play in shaping these changes, and whether this variation in miRNA expression can reveal the interplay between miRNAs and mRNAs remain poorly understood. This is especially true for miRNA expressed during key developmental transitions. Here, we assayed miRNA expression levels immediately before (>= 18BPF [18 h before puparium formation]) and after (PF) the increase in the hormone ecdysone responsible for triggering metamorphosis. We did so in four strains of Drosophila melanogaster and two closely related species. In contrast to their sequence conservation, approximately 25% of miRNAs analyzed showed significant within-species variation in male expression levels at >= 18BPF and/or PF. Additionally, approximately 33% showed modifications in their pattern of expression bias between developmental timepoints. A separate analysis of the >= 18BPF and PF stages revealed that changes in miRNA abundance accumulate linearly over evolutionary time at PF but not at >= 18BPF. Importantly, >= 18BPF-enriched miRNAs showed the greatest variation in expression levels both within and between species, so are the less likely to evolve under stabilizing selection. Functional attributes, such as expression ubiquity, appeared more tightly associated with lower levels of miRNA expression polymorphism at PF than at >= 18BPF. Furthermore, >= 18BPF- and PF-enriched miRNAs showed opposite patterns of covariation in expression with mRNAs, which denoted the type of regulatory relationship between miRNAs and mRNAs. Collectively, our results show contrasting patterns of functional divergence associated withmiRNA expression levels during Drosophila ontogeny. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity |
| 关键词[WOS] | GENE-EXPRESSION ; CODING REGIONS ; POSTTRANSCRIPTIONAL REGULATION ; CELL DIFFERENTIATION ; MICRORNA EXPRESSION ; HORMONAL-REGULATION ; IMMUNE-RESPONSE ; EVOLUTION ; MELANOGASTER ; MECHANISMS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000343402200002 |
| 公开日期 | 2015-01-20 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/20380]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Univ Calif Irvine, Dept Ecol & Evolutionary Biol, Irvine, CA 92717 USA 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China 3.Univ Sydney, Sch Math & Stat, Sydney, NSW 2006, Australia |
| 推荐引用方式 GB/T 7714 | Yeh, Shu-Dan,von Grotthuss, Marcin,Gandasetiawan, Kania A.,et al. Functional Divergence of the miRNA Transcriptome at the Onset of Drosophila Metamorphosis[J]. MOLECULAR BIOLOGY AND EVOLUTION,2014,31(10):2557-2572. |
| APA | Yeh, Shu-Dan.,von Grotthuss, Marcin.,Gandasetiawan, Kania A..,Jayasekera, Suvini.,Xia, Xiao-Qin.,...&Ranz, Jose M..(2014).Functional Divergence of the miRNA Transcriptome at the Onset of Drosophila Metamorphosis.MOLECULAR BIOLOGY AND EVOLUTION,31(10),2557-2572. |
| MLA | Yeh, Shu-Dan,et al."Functional Divergence of the miRNA Transcriptome at the Onset of Drosophila Metamorphosis".MOLECULAR BIOLOGY AND EVOLUTION 31.10(2014):2557-2572. |
入库方式： OAI收割
来源：水生生物研究所
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