A highly effective TALEN-mediated approach for targeted gene disruption in Xenopus tropicalis and zebrafish
文献类型：期刊论文
| 作者 | Liu, Yun1,4; Luo, Daji2,3; Lei, Yong1,4; Hu, Wei2; Zhao, Hui1,4; Cheng, Christopher H. K.1,4 |
| 刊名 | METHODS
 |
| 出版日期 | 2014-08-15 |
| 卷号 | 69期号:1页码:58-66 |
| 关键词 | TALENs Gene knockout Genomic deletions Xenopus Zebrafish |
| ISSN号 | 1046-2023 |
| 英文摘要 | Transcription activator like effector nucleases (TALENs) is a promising approach to disrupt intended genomic loci. The assembly of highly effective TALENs is critical for successful genome editing. Recently we reported a convenient and robust platform to construct customized TALENs. The TALENs generated by this platform have been proven to be highly effective for gene disruption in Xenopus tropicalis and zebrafish as well as large genomic deletions in zebrafish. The one-time success rate of targeted gene disruption is about 90% for more than 100 genomic loci tested, with the mutation frequencies often reaching above 50%. Here we describe the validated protocol for TALEN assembly, methods for generating gene knockout animals in X. tropicalis and zebrafish, as well as the protocol for engineering large genomic deletions in zebrafish. (C) 2014 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | ZINC-FINGER NUCLEASES ; CHROMOSOMAL DELETIONS ; GENOME ; INVERSIONS ; EMBRYOS ; SYSTEM ; GENERATION ; CRISPR/CAS ; TOOLBOX ; CAS9 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000341803500009 |
| 公开日期 | 2015-01-20 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/20346]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Univ Hong Kong, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China 3.Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430072, Peoples R China 4.Chinese Univ Hong Kong, Shenzhen Res Inst, Sch Biomed Sci, Core Lab, Shenzhen 518057, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yun,Luo, Daji,Lei, Yong,et al. A highly effective TALEN-mediated approach for targeted gene disruption in Xenopus tropicalis and zebrafish[J]. METHODS,2014,69(1):58-66. |
| APA | Liu, Yun,Luo, Daji,Lei, Yong,Hu, Wei,Zhao, Hui,&Cheng, Christopher H. K..(2014).A highly effective TALEN-mediated approach for targeted gene disruption in Xenopus tropicalis and zebrafish.METHODS,69(1),58-66. |
| MLA | Liu, Yun,et al."A highly effective TALEN-mediated approach for targeted gene disruption in Xenopus tropicalis and zebrafish".METHODS 69.1(2014):58-66. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。