Growth and Nitrogen Uptake Characteristics Reveal Outbreak Mechanism of the Opportunistic Macroalga Gracilaria tenuistipitata
文献类型：期刊论文
| 作者 | Wang, Chao1,2; Lei, Anping1; Zhou, Kai3; Hu, Zhengyu2; Hao, Wenlong1; Yang, Junda1 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2014-10-09 |
| 卷号 | 9期号:10 |
| 关键词 | NITRATE UPTAKE INORGANIC NITROGEN COASTAL WATERS ULVA-LACTUCA INTERTIDAL SEAWEEDS NUTRIENT CONTROL AMMONIUM UPTAKE UPTAKE KINETICS AGAR YIELD J. AGARDH |
| ISSN号 | 1932-6203 |
| 英文摘要 | Macroalgae has bloomed in the brackish lake of Shenzhen Bay, China continuously from 2010 to 2014. Gracilaria tenuistipitata was identified as the causative macroalgal species. The aim of this study was to explore the outbreak mechanism of G. tenuistipitata, by studying the effects of salinity and nitrogen sources on growth, and the different nitrogen sources uptake characteristic. Our experimental design was based on environmental conditions observed in the bloom areas, and these main factors were simulated in the laboratory. Results showed that salinity 12 to 20 % was suitable for G. tenuistipitata growth. When the nitrogen sources' (NH4+, NO3+) concentrations reached 40 mu M or above, the growth rate of G. tenuistipitata was significantly higher. Algal biomass was higher (approximately 1.4 times) when cultured with NH4+ than that with NO3+ addition. Coincidentally, macroalgal bloom formed during times of moderate salinity (similar to 12 %) and high nitrogen conditions. The NH4+ and NO3+ uptake characteristic was studied to understand the potential mechanism of G. tenuistipitata bloom. NH4+ uptake was best described by a linear, rate-unsaturated response, with the slope decreasing with time intervals. In contrast, NO3+ uptake followed a rate-saturating mechanism best described by the Michaelis-Menten model, with kinetic parameters V-max = 37.2 mu M g(-1) DM h(-1) and K-s = 61.5 mu M. Further, based on the isotope N-15 tracer method, we found that N-15 from NH4+ accumulated faster and reached an atom% twice than that of N-15 from NO3+, suggesting when both NH4+ and NO3+ were available, NH4+ was assimilated more rapidly. The results of the present study indicate that in the estuarine environment, the combination of moderate salinity with high ammonium may stimulate bloom formation. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | NITRATE UPTAKE ; INORGANIC NITROGEN ; COASTAL WATERS ; ULVA-LACTUCA ; INTERTIDAL SEAWEEDS ; NUTRIENT CONTROL ; AMMONIUM UPTAKE ; UPTAKE KINETICS ; AGAR YIELD ; J. AGARDH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000343941200027 |
| 公开日期 | 2015-01-20 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/20394]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | 1.Shenzhen Univ, Coll Life Sci, Shenzhen, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China 3.Shenzhen Marine Environm & Resources Monitoring C, Shenzhen, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Chao,Lei, Anping,Zhou, Kai,et al. Growth and Nitrogen Uptake Characteristics Reveal Outbreak Mechanism of the Opportunistic Macroalga Gracilaria tenuistipitata[J]. PLOS ONE,2014,9(10). |
| APA | Wang, Chao,Lei, Anping,Zhou, Kai,Hu, Zhengyu,Hao, Wenlong,&Yang, Junda.(2014).Growth and Nitrogen Uptake Characteristics Reveal Outbreak Mechanism of the Opportunistic Macroalga Gracilaria tenuistipitata.PLOS ONE,9(10). |
| MLA | Wang, Chao,et al."Growth and Nitrogen Uptake Characteristics Reveal Outbreak Mechanism of the Opportunistic Macroalga Gracilaria tenuistipitata".PLOS ONE 9.10(2014). |
入库方式： OAI收割
来源：水生生物研究所
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