High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation
文献类型：期刊论文
| 作者 | Zhang, Chunye1,2; Hu, Hanhua1 |
| 刊名 | MARINE GENOMICS
 |
| 出版日期 | 2014-08-01 |
| 卷号 | 16页码:63-66 |
| 关键词 | Electroporation Diatom transformation Cotransformation eGFP GUS |
| ISSN号 | 1874-7787 |
| 英文摘要 | We established a high-efficiency nuclear transformation method for the diatom Phaeodactylum tricornutum using an electroporation system. Based on a universal electroporation protocol, the conditions for the introduction of exogenous DNA including electric field strength and plasmid form were optimized. Following optimization, the diatom cells could be transformed with exogenous gene easily, the maximum transformation frequency obtained was 2.8 x 10(-5) cells. The cotransformation of P. tricornutum with a non-selective GUS gene together with the selectable resistance gene has also been achieved using our new method and found to be very efficient (up to 60%). The electroporation procedure described in this article offers a number of advantages, including simplicity, general utility, low-cost and high efficiency. The described method also provides some clue for developing electroporation transformation system in other eukaryotic microalgae. (C) 2013 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Genetics & Heredity ; Marine & Freshwater Biology |
| 研究领域[WOS] | Genetics & Heredity ; Marine & Freshwater Biology |
| 关键词[WOS] | GENETIC-TRANSFORMATION ; CYLINDROTHECA-FUSIFORMIS ; REPORTER GENES ; BACILLARIOPHYCEAE ; ESTABLISHMENT ; PROMOTER ; MARKER ; SYSTEM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000343392100008 |
| 公开日期 | 2015-01-20 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/20402]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Chunye,Hu, Hanhua. High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation[J]. MARINE GENOMICS,2014,16:63-66. |
| APA | Zhang, Chunye,&Hu, Hanhua.(2014).High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation.MARINE GENOMICS,16,63-66. |
| MLA | Zhang, Chunye,et al."High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation".MARINE GENOMICS 16(2014):63-66. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。