High-efficiency nuclear transformation of the oleaginous marine Nannochloropsis species using PCR product
文献类型：期刊论文
| 作者 | Li, Fengjuan1; Gao, Dawen1; Hu, Hanhua2 |
| 刊名 | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
 |
| 出版日期 | 2014-05-01 |
| 卷号 | 78期号:5页码:812-817 |
| 关键词 | electroporation Nannochloropsis transformation cotransformation GUS |
| ISSN号 | 0916-8451 |
| 英文摘要 | Nannochloropsis are model species for investigating biofuel production by algae. To develop them into an integrated photons-to-fuel production platform, high efficiency transformation methods are necessary. Here, we obtained the beta-tubulin promoter regions of all recognized species of genus Nannochloropsis, and successfully transformed all five marine species by electroporation. In addition, the PCR amplified double stranded DNA fragments (PCR fragments) based transformation system was established in these Nannochloropsis species, which showed much higher transformation efficiency (10.7-61.2 x 10 (6), 1.5-13-fold) than that of linearized plasmid based transformation. The cotransformation of N. salina using a circular plasmid containing a non-selectable GUS gene and a PCR fragment containing only a selection marker cassette was also achieved and found to be very efficient (over 50%). This simple and highly efficient transformation protocol reported in our study provided a useful tool for gene functional analysis and genetic engineering of the oleaginous Nannochloropsis species. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry, Applied ; Food Science & Technology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry ; Food Science & Technology |
| 关键词[WOS] | DIATOM PHAEODACTYLUM-TRICORNUTUM ; FATTY-ACID-COMPOSITION ; MICROALGAE ; GROWTH ; EUSTIGMATOPHYCEAE ; TRIACYLGLYCEROLS ; CHLAMYDOMONAS ; AQUACULTURE ; GENES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000339070600014 |
| 公开日期 | 2015-01-20 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/20406]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | 1.Harbin Inst Technol, State Key Lab Urban Water Resource & Environm, Harbin 150006, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Fengjuan,Gao, Dawen,Hu, Hanhua. High-efficiency nuclear transformation of the oleaginous marine Nannochloropsis species using PCR product[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2014,78(5):812-817. |
| APA | Li, Fengjuan,Gao, Dawen,&Hu, Hanhua.(2014).High-efficiency nuclear transformation of the oleaginous marine Nannochloropsis species using PCR product.BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,78(5),812-817. |
| MLA | Li, Fengjuan,et al."High-efficiency nuclear transformation of the oleaginous marine Nannochloropsis species using PCR product".BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 78.5(2014):812-817. |
入库方式： OAI收割
来源：水生生物研究所
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