Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutum
文献类型：期刊论文
| 作者 | Ge, Feng1; Huang, Weichao2; Chen, Zhuo2; Zhang, Chunye2; Xiong, Qian1; Bowler, Chris3; Yang, Juan2; Xu, Jin2; Hu, Hanhua1,2 |
| 刊名 | PLANT CELL
 |
| 出版日期 | 2014-04-01 |
| 卷号 | 26期号:4页码:1681-1697 |
| 关键词 | MICROALGA CHLAMYDOMONAS-REINHARDTII LIPID-ACCUMULATION NITROGEN DEPRIVATION MASS-SPECTROMETRY OIL ACCUMULATION BRASSICA-NAPUS MALIC ENZYME UREA CYCLE METABOLISM QUANTIFICATION |
| ISSN号 | 1040-4651 |
| 英文摘要 | The model marine diatom Phaeodactylum tricornutum can accumulate high levels of triacylglycerols (TAGs) under nitrogen depletion and has attracted increasing attention as a potential system for biofuel production. However, the molecular mechanisms involved in TAG accumulation in diatoms are largely unknown. Here, we employed a label-free quantitative proteomics approach to estimate differences in protein abundance before and after TAG accumulation. We identified a total of 1193 proteins, 258 of which were significantly altered during TAG accumulation. Data analysis revealed major changes in proteins involved in branched-chain amino acid (BCAA) catabolic processes, glycolysis, and lipid metabolic processes. Subsequent quantitative RT-PCR and protein gel blot analysis confirmed that four genes associated with BCAA degradation were significantly upregulated at both the mRNA and protein levels during TAG accumulation. The most significantly upregulated gene, encoding the beta-subunit of methylcrotonyl-CoA carboxylase (MCC2), was selected for further functional studies. Inhibition of MCC2 expression by RNA interference disturbed the flux of carbon (mainly in the form of leucine) toward BCAA degradation, resulting in decreased TAG accumulation. MCC2 inhibition also gave rise to incomplete utilization of nitrogen, thus lowering biomass during the stationary growth phase. These findings help elucidate the molecular and metabolic mechanisms leading to increased lipid production in diatoms. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| 关键词[WOS] | MICROALGA CHLAMYDOMONAS-REINHARDTII ; LIPID-ACCUMULATION ; NITROGEN DEPRIVATION ; MASS-SPECTROMETRY ; OIL ACCUMULATION ; BRASSICA-NAPUS ; MALIC ENZYME ; UREA CYCLE ; METABOLISM ; QUANTIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000337351300023 |
| 公开日期 | 2015-01-20 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/20408]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Diatom Biol Grp, Wuhan 430072, Peoples R China 3.Ecole Normale Super, CNRS, Unite Mixte Rech 8197,U1024, INSERM,Environm & Evolutionary Genom Sect,Inst Bi, F-75230 Paris 05, France |
| 推荐引用方式 GB/T 7714 | Ge, Feng,Huang, Weichao,Chen, Zhuo,et al. Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutum[J]. PLANT CELL,2014,26(4):1681-1697. |
| APA | Ge, Feng.,Huang, Weichao.,Chen, Zhuo.,Zhang, Chunye.,Xiong, Qian.,...&Hu, Hanhua.(2014).Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutum.PLANT CELL,26(4),1681-1697. |
| MLA | Ge, Feng,et al."Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutum".PLANT CELL 26.4(2014):1681-1697. |
入库方式： OAI收割
来源：水生生物研究所
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