Quantification of multiple compounds containing heterogeneous elements in the mixture by one-dimensional nuclear magnetic resonance spectroscopy of different nuclei using a single universal concentration reference
文献类型：期刊论文
| 作者 | Xu, Li; Shi, Xiaohuo; Hu, Kaifeng
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| 刊名 | MAGNETIC RESONANCE IN CHEMISTRY
 |
| 出版日期 | 2014-12-01 |
| 卷号 | 52期号:12页码:779-782 |
| 关键词 | joint 1D qNMR universal concentration reference trimethylphosphine oxide |
| ISSN号 | 0749-1581 |
| 产权排序 | 第一 |
| 通讯作者 | Hu,KF (reprint author),Chinese Acad Sci,State Key Lab Phytochem & Plant Resources West Ch,Kunming Inst Bot,132 Lanhei Rd,Kunming 650201,Yunnan,Peoples R China. ; kaifenghu@mail.kib.ac.cn |
| 英文摘要 | One-dimensional (1D) quantitative NMR (qNMR) is a useful tool for concentration determination due to its experimental simplicity and the direct proportionality of the integrated signal area to the number of nuclei spin. For complex mixtures, however, signal overlapping often in one-dimensional quantitative H-1 NMR (1D H-1 qNMR) spectrum limits the accurate quantification of individual compound. Here, we introduced employing joint 1D qNMR methods of different nuclei, such as H-1 and P-31 (or/and F-19), to quantify multiple compounds in a complex mixture using a single universal concentration reference. When the concentration ratio of several compounds containing different elements in a complex mixture is of interest, the result calculated from measured intensities from 1D qNMR of different nuclei is independent of the gravimetric error from the reference. In this case, the common reference also serves as a quantitative bridge' among these 1D qNMR of different nuclei. Quantitative analysis of choline, phosphocholine, and glycerophosphocholine mixture is given as an example using trimethylphosphine oxide ((CH3)(3)P(O)) as concentration reference. Compounds containing multiple elements, such as tetramethylammonium hexafluorophosphate (N+(CH3)(4)PF6-), are proposed as the common concentration reference for H-1, C-13, N-15, P-31, and F-19 qNMR for the quantitative analysis of complex mixture containing these different elements. We anticipate that the proposed joint 1D qNMR approach using a universal concentration reference will be a valuable alternative for simultaneous quantification of multiple compounds in a complex mixture due to its accuracy and single and simple sample preparation. Copyright (c) 2014 John Wiley & Sons, Ltd. |
| 学科主题 | Chemistry, Multidisciplinary;Chemistry, Physical;Spectroscopy |
| 类目[WOS] | Chemistry, Multidisciplinary ; Chemistry, Physical ; Spectroscopy |
| 研究领域[WOS] | Chemistry ; Spectroscopy |
| 关键词[WOS] | NMR-SPECTROSCOPY ; H-1-NMR ; METABOLITES |
| 收录类别 | SCI |
| 资助信息 | Major State Basic Research Development Program [2010CB951700]; Young Academic and Technical Leader Raising Foundation of Yunnan Province [2012HB041]; National Science Foundation of China [31170256, 31260167] |
| 语种 | 英语 |
| WOS记录号 | WOS:000345509000007 |
| 公开日期 | 2015-01-20 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/18458]  |
| 专题 | 昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | Chinese Acad Sci, State Key Lab Phytochem & Plant Resources West Ch, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Li,Shi, Xiaohuo,Hu, Kaifeng. Quantification of multiple compounds containing heterogeneous elements in the mixture by one-dimensional nuclear magnetic resonance spectroscopy of different nuclei using a single universal concentration reference[J]. MAGNETIC RESONANCE IN CHEMISTRY,2014,52(12):779-782. |
| APA | Xu, Li,Shi, Xiaohuo,&Hu, Kaifeng.(2014).Quantification of multiple compounds containing heterogeneous elements in the mixture by one-dimensional nuclear magnetic resonance spectroscopy of different nuclei using a single universal concentration reference.MAGNETIC RESONANCE IN CHEMISTRY,52(12),779-782. |
| MLA | Xu, Li,et al."Quantification of multiple compounds containing heterogeneous elements in the mixture by one-dimensional nuclear magnetic resonance spectroscopy of different nuclei using a single universal concentration reference".MAGNETIC RESONANCE IN CHEMISTRY 52.12(2014):779-782. |
入库方式： OAI收割
来源：昆明植物研究所
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