IRDL Cloning: A One-Tube, Zero-Background, Easy-to-Use, Directional Cloning Method Improves Throughput in Recombinant DNA Preparation
文献类型：期刊论文
| 作者 | Wang, Jiancai1,2; Xu, Ronghua2; Liu, Aizhong2,3
|
| 刊名 | PLOS ONE
 |
| 出版日期 | 2014-09-22 |
| 卷号 | 9期号:9页码:8893-8899 |
| 关键词 | BRAZILIAN AMAZON OIL PALM CHINA RUBBER XISHUANGBANNA EXPANSION TRANSFORMATION DEFORESTATION RESILIENCE DYNAMICS Land use Deforestation Prediction REDD Sustainability Southeast Asia |
| ISSN号 | 1932-6203 |
| 产权排序 | 第二 |
| 通讯作者 | Xu,RH (reprint author),Chinese Acad Sci,Xishuangbanna Trop Bot Garden,Key Lab Trop Plant Resources & Sustainable Use,Kunming,Peoples R China. ; ronghua.xu08@gmail.com ; aizhongliu@xtbg.ac.cn |
| 英文摘要 | Rapid and efficient construction of expression vectors and subsequent transformation are basic recombinant methods for the investigation of gene functionality. Although novel cloning methods have recently been developed, many laboratories worldwide continue to use traditional restriction digestion-ligation methods to construct expression vectors owing to financial constraints and the unavailability of appropriate vectors. We describe an improved restriction digestion-ligation (IRDL) cloning method that combines the advantage of directional cloning from double digestion-ligation with that of a low background observed by using a positive selection marker gene ccdB to facilitate digestion and ligation in a single tube. The IRDL cloning overcomes the time-consuming and laborious limits of traditional methods, thereby providing an easy-to-use, low-cost, and one-step strategy for directional cloning of target DNA fragments into an expression vector. As a proof-of-concept example, we developed two yeast vectors to demonstrate the feasibility and the flexibility of the IRDL cloning method. This method would provide an effective and easy-to-use system for gene cloning and functional genomics studies. |
| 学科主题 | Multidisciplinary Sciences |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | LIGATION-INDEPENDENT CLONING ; PLANT FUNCTIONAL GENOMICS ; TOXIN-ANTITOXIN SYSTEMS ; ONE-STEP ; PCR PRODUCTS ; POSITIVE-SELECTION ; ESCHERICHIA-COLI ; VECTOR ; TRANSFORMATION ; GENE |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [90813004, 2009312311024]; National Basic Research Program of China (973 Program) [2011CB915503, 2009CB522303] |
| 语种 | 英语 |
| WOS记录号 | WOS:000343679800056 |
| 公开日期 | 2015-01-20 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/18507]  |
| 专题 | 昆明植物研究所_资源植物与生物技术所级重点实验室 |
| 作者单位 | 1.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Peoples R China 2.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Key Lab Trop Plant Resources & Sustainable Use, Kunming, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jiancai,Xu, Ronghua,Liu, Aizhong. IRDL Cloning: A One-Tube, Zero-Background, Easy-to-Use, Directional Cloning Method Improves Throughput in Recombinant DNA Preparation[J]. PLOS ONE,2014,9(9):8893-8899. |
| APA | Wang, Jiancai,Xu, Ronghua,&Liu, Aizhong.(2014).IRDL Cloning: A One-Tube, Zero-Background, Easy-to-Use, Directional Cloning Method Improves Throughput in Recombinant DNA Preparation.PLOS ONE,9(9),8893-8899. |
| MLA | Wang, Jiancai,et al."IRDL Cloning: A One-Tube, Zero-Background, Easy-to-Use, Directional Cloning Method Improves Throughput in Recombinant DNA Preparation".PLOS ONE 9.9(2014):8893-8899. |
入库方式： OAI收割
来源：昆明植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。