Production of allitol from D-psicose by a novel isolated strain of Klebsiella oxytoca G4A4
文献类型:期刊论文
作者 | Han, Wenjia; Zhu, Yueming; Men, Yan; Yang, Jiangang; Liu, Can; Sun, Yuanxia |
刊名 | JOURNAL OF BASIC MICROBIOLOGY
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出版日期 | 2014-10-01 |
卷号 | 54期号:10页码:1073-1079 |
关键词 | Allitol Biotransformation D-psicose Klebsiella oxytoca |
英文摘要 | A novel bacterium capable of producing allitol from D-psicose was isolated from soil and identified as Klebsiella oxytoca G4A4. An efficient method for the transformation of D-psicose to allitol was achieved through the resting cell reaction. Ribitol as an inducer is suitable for cell cultivation, and cells are most active in Tris-HCl buffer (pH 8.0) at 37 degrees C with a density of 40 (OD600nm). After the reaction, the final conversion rates of the washed cells were approximately 87, 83, and 55% at D-psicose concentrations of 0.25, 0.5, and 1%, respectively. The product from D-psicose was purified and determined to be allitol by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Microbiology |
研究领域[WOS] | Microbiology |
关键词[WOS] | RIBITOL DEHYDROGENASE ; ESCHERICHIA-COLI ; RARE SUGARS ; D-ALLOSE ; BIOPRODUCTION ; 3-EPIMERASE ; AEROGENES ; PURIFICATION ; STRATEGY ; XYLITOL |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000343816700007 |
公开日期 | 2015-01-24 |
源URL | [http://124.16.173.210/handle/311007/697] ![]() |
专题 | 天津工业生物技术研究所_功能糖与天然活性物质研究组 孙媛霞 _期刊论文 |
作者单位 | Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China |
推荐引用方式 GB/T 7714 | Han, Wenjia,Zhu, Yueming,Men, Yan,et al. Production of allitol from D-psicose by a novel isolated strain of Klebsiella oxytoca G4A4[J]. JOURNAL OF BASIC MICROBIOLOGY,2014,54(10):1073-1079. |
APA | Han, Wenjia,Zhu, Yueming,Men, Yan,Yang, Jiangang,Liu, Can,&Sun, Yuanxia.(2014).Production of allitol from D-psicose by a novel isolated strain of Klebsiella oxytoca G4A4.JOURNAL OF BASIC MICROBIOLOGY,54(10),1073-1079. |
MLA | Han, Wenjia,et al."Production of allitol from D-psicose by a novel isolated strain of Klebsiella oxytoca G4A4".JOURNAL OF BASIC MICROBIOLOGY 54.10(2014):1073-1079. |
入库方式: OAI收割
来源:天津工业生物技术研究所
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