中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Production of allitol from D-psicose by a novel isolated strain of Klebsiella oxytoca G4A4

文献类型:期刊论文

作者Han, Wenjia; Zhu, Yueming; Men, Yan; Yang, Jiangang; Liu, Can; Sun, Yuanxia
刊名JOURNAL OF BASIC MICROBIOLOGY
出版日期2014-10-01
卷号54期号:10页码:1073-1079
关键词Allitol Biotransformation D-psicose Klebsiella oxytoca
英文摘要A novel bacterium capable of producing allitol from D-psicose was isolated from soil and identified as Klebsiella oxytoca G4A4. An efficient method for the transformation of D-psicose to allitol was achieved through the resting cell reaction. Ribitol as an inducer is suitable for cell cultivation, and cells are most active in Tris-HCl buffer (pH 8.0) at 37 degrees C with a density of 40 (OD600nm). After the reaction, the final conversion rates of the washed cells were approximately 87, 83, and 55% at D-psicose concentrations of 0.25, 0.5, and 1%, respectively. The product from D-psicose was purified and determined to be allitol by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Microbiology
研究领域[WOS]Microbiology
关键词[WOS]RIBITOL DEHYDROGENASE ; ESCHERICHIA-COLI ; RARE SUGARS ; D-ALLOSE ; BIOPRODUCTION ; 3-EPIMERASE ; AEROGENES ; PURIFICATION ; STRATEGY ; XYLITOL
收录类别SCI
语种英语
WOS记录号WOS:000343816700007
公开日期2015-01-24
源URL[http://124.16.173.210/handle/311007/697]  
专题天津工业生物技术研究所_功能糖与天然活性物质研究组 孙媛霞 _期刊论文
作者单位Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China
推荐引用方式
GB/T 7714
Han, Wenjia,Zhu, Yueming,Men, Yan,et al. Production of allitol from D-psicose by a novel isolated strain of Klebsiella oxytoca G4A4[J]. JOURNAL OF BASIC MICROBIOLOGY,2014,54(10):1073-1079.
APA Han, Wenjia,Zhu, Yueming,Men, Yan,Yang, Jiangang,Liu, Can,&Sun, Yuanxia.(2014).Production of allitol from D-psicose by a novel isolated strain of Klebsiella oxytoca G4A4.JOURNAL OF BASIC MICROBIOLOGY,54(10),1073-1079.
MLA Han, Wenjia,et al."Production of allitol from D-psicose by a novel isolated strain of Klebsiella oxytoca G4A4".JOURNAL OF BASIC MICROBIOLOGY 54.10(2014):1073-1079.

入库方式: OAI收割

来源:天津工业生物技术研究所

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