Evidence of a Critical Role for Cellodextrin Transporte 2 (CDT-2) in Both Cellulose and Hemicellulose Degradation and Utilization in Neurospora crassa
文献类型：期刊论文
| 作者 | Cai, Pengli1; Gu, Ruimeng1; Wang, Bang1; Li, Jingen1; Wan, Li1; Tian, Chaoguang1; Ma, Yanhe1,2 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2014-02-20 |
| 卷号 | 9期号:2 |
| 英文摘要 | CDT-1 and CDT-2 are two cellodextrin transporters discovered in the filamentous fungus Neurospora crassa. Previous studies focused on characterizing the role of these transporters in only a few conditions, including cellulose degradation, and the function of these two transporters is not yet completely understood. In this study, we show that deletion of cdt-2, but not cdt-1, results in growth defects not only on Avicel but also on xylan. cdt-2 can be highly induced by xylan, and this mutant has a xylodextrin consumption defect. Transcriptomic analysis of the cdt-2 deletion strain on Avicel and xylan showed that major cellulase and hemicellulase genes were significantly down-regulated in the cdt-2 deletion strain and artificial over expression of cdt-2 in N. crassa increased cellulase and hemicellulase production. Together, these data clearly show that CDT-2 plays a critical role in hemicellulose sensing and utilization. This is the first time a sugar transporter has been assigned a function in the hemicellulose degradation pathway. Furthermore, we found that the transcription factor XLR-1 is the major regulator of cdt-2, while cdt-1 is primarily regulated by CLR-1. These results deepen our understanding of the functions of both cellodextrin transporters, particularly for CDT-2. Our study also provides novel insight into the mechanisms for hemicellulose sensing and utilization in N. crassa, and may be applicable to other cellulolytic filamentous fungi. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | TRICHODERMA-REESEI ; RNA-SEQ ; CATABOLITE REPRESSION ; INDUCTION ; HYDROLYSIS ; EXPRESSION ; XYLANASE ; FUNGUS ; GENE ; MECHANISMS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000331714700103 |
| 公开日期 | 2015-01-24 |
| 源URL | [http://124.16.173.210/handle/311007/695]  |
| 专题 | 天津工业生物技术研究所_微生物功能基因组学与生物技术实验室 田朝光_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cai, Pengli,Gu, Ruimeng,Wang, Bang,et al. Evidence of a Critical Role for Cellodextrin Transporte 2 (CDT-2) in Both Cellulose and Hemicellulose Degradation and Utilization in Neurospora crassa[J]. PLOS ONE,2014,9(2). |
| APA | Cai, Pengli.,Gu, Ruimeng.,Wang, Bang.,Li, Jingen.,Wan, Li.,...&Ma, Yanhe.(2014).Evidence of a Critical Role for Cellodextrin Transporte 2 (CDT-2) in Both Cellulose and Hemicellulose Degradation and Utilization in Neurospora crassa.PLOS ONE,9(2). |
| MLA | Cai, Pengli,et al."Evidence of a Critical Role for Cellodextrin Transporte 2 (CDT-2) in Both Cellulose and Hemicellulose Degradation and Utilization in Neurospora crassa".PLOS ONE 9.2(2014). |
入库方式： OAI收割
来源：天津工业生物技术研究所
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