High-Frequency Regeneration by Abscisic Acid (ABA) from Petiole Callus of Jatropha curcas
文献类型：期刊论文
| 作者 | Singh, A. |
| 刊名 | In Vitro Cellular & Developmental Biology-Plant
 |
| 出版日期 | 2014 |
| 卷号 | 50期号:5页码:638-645 |
| ISSN号 | 1054-5476 |
| 中文摘要 | Jatropha curcas L. is attaining worldwide interest as an important biofuel crop. Experiments were conducted to improve the prevailing micropropagation technique as well as to develop a new ex vitro rooting method for J. curcas plant regeneration. Regeneration and ex vitro rooting efficiency was enhanced by augmenting the culture medium with abscisic acid (ABA). Different concentrations of 6-benzylaminopurine (BAP) and indole-3-butyric acid (IBA) were tested for callus generation from both in vitro and in vivo explants (leaf and petiole) on Murashige and Skoog (MS) medium. The best regenerative callus was achieved on MS medium supplemented with BAP (4.44 mu M) and IBA (2.45 mu M) from in vitro-cultured petioles. Highest regeneration (91%) was achieved by culturing petiole callus on MS medium supplemented with BAP (8.88 mu M), IBA (0.49 mu M), and ABA (1.9 mu M), whereas 61% regeneration was obtained from in vitro leaf callus. Shoot proliferation and elongation was achieved on BAP (2.22 mu M) and IAA (8.56 mu M) with 10-13 shoots per explants. Highest rooting (65%) was achieved from M1 shoots (BAP, IAA, and ABA) on MS medium supplemented with IBA (2.45 mu M), naphthaleneacetic acid NAA (0.54 mu M), and 0.02% activated charcoal. Ex vitro rooting of 1-mo-old M1 shoots obtained from the charcoal-containing medium resulted optimum rooting (>72%) when transferred to polybags containing sterile sand. The plantlets were successfully acclimatized in soil with more than 98% survival rate in the greenhouse. |
| 公开日期 | 2015-01-28 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/7168]  |
| 专题 | 西双版纳热带植物园_热带植物资源可持续利用重点实验室特色文献_小桐子相关文献 |
| 推荐引用方式 GB/T 7714 | Singh, A.. High-Frequency Regeneration by Abscisic Acid (ABA) from Petiole Callus of Jatropha curcas[J]. In Vitro Cellular & Developmental Biology-Plant,2014,50(5):638-645. |
| APA | Singh, A..(2014).High-Frequency Regeneration by Abscisic Acid (ABA) from Petiole Callus of Jatropha curcas.In Vitro Cellular & Developmental Biology-Plant,50(5),638-645. |
| MLA | Singh, A.."High-Frequency Regeneration by Abscisic Acid (ABA) from Petiole Callus of Jatropha curcas".In Vitro Cellular & Developmental Biology-Plant 50.5(2014):638-645. |
入库方式： OAI收割
来源：西双版纳热带植物园
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