Esterase and lipase activity in Jatropha curcas L-seeds
文献类型：期刊论文
| 作者 | Staubmann, R. |
| 刊名 | Journal of Biotechnology
 |
| 出版日期 | 1999 |
| 卷号 | 75期号:42038页码:117-126 |
| ISSN号 | 0168-1656 |
| 中文摘要 | Two new esterases (JEA and JEB) and a lipase (JL) were extracted from the seeds of Jatropha curcas L. Lipase activity was only found during germination of the seeds and increased to a maximum after 4 days of germination. All enzymes were found to be most active in the alkaline range at around pH 8 and the purified (fractionated precipitation with ethanol and gel filtration) esterases were very stable at high temperatures. The molecular weight (SDS-PAGE) of both esterases was determined to be 21.6-23.5 kDa (JEA) and 30.2 kDa (JEB) and the isoelectric point was 5.7-6.1 for esterase JEA and 9.0 for esterase JEB. Most ions caused a negative influence on the activity of both esterases. Using p-nitrophenyl butyrate as a substrate JEA showed a K-m of 0.02 mM and a v(max) of 0.26 mu mol mg(-1) min(-1). Under the same conditions JEB showed a K-m of 0.07 mM and a v(max) of 0.24 mu mol mg(-1) min(-1). Both esterases hydrolyzed tributyrin, nitrophenyl esters up to a chain length of =C4 and naphtylesters up to a chain length =C6. In transesterification reactions, JL was found to be most active at very low water activities (0.2) and in high water activities, the lipase hydrolysed triglycerides into conversions above 80%. The lipase hydrolysed both short chain and long chain triglycerides at about the same rate but was inactive on alpha-methylbenzyl acetate. JL is a potentially useful biocatalyst in the hydrolysis of triglycerides in organic solvents. (C) 1999 Elsevier Science B.V. All rights reserved. |
| 公开日期 | 2015-01-28 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/7869]  |
| 专题 | 西双版纳热带植物园_热带植物资源可持续利用重点实验室特色文献_小桐子相关文献 |
| 推荐引用方式 GB/T 7714 | Staubmann, R.. Esterase and lipase activity in Jatropha curcas L-seeds[J]. Journal of Biotechnology,1999,75(42038):117-126. |
| APA | Staubmann, R..(1999).Esterase and lipase activity in Jatropha curcas L-seeds.Journal of Biotechnology,75(42038),117-126. |
| MLA | Staubmann, R.."Esterase and lipase activity in Jatropha curcas L-seeds".Journal of Biotechnology 75.42038(1999):117-126. |
入库方式： OAI收割
来源：西双版纳热带植物园
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